Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, New Delhi, 110016, India.
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, New Delhi, 110016, India.
J Biotechnol. 2018 Dec 10;287:59-67. doi: 10.1016/j.jbiotec.2018.10.004. Epub 2018 Oct 12.
Biodesulfurization is an eco-friendly process for removing sulfur from petroleum fractions. The process could not be commercialized because of the inability of microorganisms to desulfurize a wide range of heterocyclic poly aromatic sulfur compounds like dibenzothiophene (DBT), 4, 6-Dimethyl DBT present in fuel and low desulfurization activity. In the present study, to improve the rates of conversion of dibenzothiophene to dibenzothiophene sulfone, the responsible enzyme dibenzothiophene monooxygenase DszC, is displayed on the surface of Escherichia coli. This helped in overcoming the mass transfer limitation and resulted in approximately 3 times faster conversion with respect to control (which contained intracellular enzyme). This is the first report demonstrating display of a biodesulfurization enzyme on bacterial cell surface.
生物脱硫是一种从石油馏分中去除硫的环保工艺。由于微生物无法脱硫，该工艺无法商业化，这些无法脱硫的物质包括像二苯并噻吩（DBT）、燃料中的 4,6-二甲基二苯并噻吩（4,6-Dimethyl DBT）等广泛的杂环多芳烃硫化合物和低脱硫活性。在本研究中，为了提高二苯并噻吩转化为二苯并噻吩砜的速率，将负责的酶二苯并噻吩单加氧酶 DszC 展示在大肠杆菌的表面。这有助于克服质量传递限制，使转化率相对于对照（含有细胞内酶）提高了约 3 倍。这是首次证明在细菌细胞表面展示生物脱硫酶的报告。
