Division of Food and Nutrition, Wakayama Shin-Ai Women's Junior College, Wakayama, Japan.
Wakayama Medical University, Wakayama, Japan.
Microbiol Immunol. 2019 Sep;63(9):359-366. doi: 10.1111/1348-0421.12729. Epub 2019 Aug 22.
Umesu phenolics were obtained from the salt extracts of Japanese apricot (Nanko-mume cultivar of Prunus mume Sieb. et Zucc.) as purified phenolics. The antiviral activities of umesu phenolics obtained were then examined against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), enveloped DNA viruses. The phenolics inhibited the multiplication of these viruses when added to the culture media of the infected cells. This inhibition occurred at phenolic concentrations at which they showed no severe cytotoxicity. One-step growth experiments showed that the eclipse period in the HSV-1 multiplication process was extended in the presence of umesu phenolics and that the addition of phenolics after the completion of viral DNA replication did not affect their multiplication. More drastic effects were observed on virucidal activities against HSV-1 and HSV-2; the infectivity decreased to 0.0001 when infected cells were incubated with 3 mg/ml phenolics at 30°C for 5 min. These results demonstrate the antiviral and virucidal activities of umesu phenolics and suggest a potential pharmacological use for these phenolics as a sanitizing or preventive medicine against superficial HSV infections.
梅苏酚类物质是从日本甜樱桃（Prunus mume Sieb. et Zucc. 的 Nanko-mume 品种）的盐提取物中获得的纯化酚类物质。然后，研究了获得的梅苏酚类物质对单纯疱疹病毒 1 型（HSV-1）和 2 型（HSV-2）这两种包膜 DNA 病毒的抗病毒活性。当将酚类物质添加到受感染细胞的培养基中时，它们可以抑制这些病毒的繁殖。这种抑制作用发生在酚类物质浓度下，它们没有表现出严重的细胞毒性。一步生长实验表明，在梅苏酚类物质存在下，HSV-1 增殖过程中的潜伏期延长，并且在病毒 DNA 复制完成后添加酚类物质不会影响其增殖。对 HSV-1 和 HSV-2 的杀病毒活性观察到更剧烈的影响；当用 3mg/ml 的酚类物质在 30°C 下孵育感染细胞 5 分钟时，感染性降低至 0.0001。这些结果表明梅苏酚类物质具有抗病毒和杀病毒活性，并表明这些酚类物质作为针对浅表性 HSV 感染的消毒或预防药物具有潜在的药理学用途。
