Ferreira Daniela, Soares Maria, Correia Jorge, Adega Filomena, Ferreira Fernando, Chaves Raquel
CAG - Laboratory of Cytogenomics and Animal Genomics, Department of Genetics and Biotechnology, University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal.
BioISI - Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, Lisbon 1749-016, Portugal.
Aging (Albany NY). 2019 Jul 12;11(13):4688-4705. doi: 10.18632/aging.102079.
In humans, the gene amplification and overexpression are biomarkers for invasive breast cancer and a therapeutic target. Also, gene aberrations predict the response to anthracycline-based adjuvant chemotherapy. Although feline mammary tumors (FMTs) are good models in comparative oncology, scarce data is available regarding the and status. In this study, and for the first time, the DNA status and RNA levels of intracellular (ICD) and extracellular (ECD) coding regions were compared with gene status and expression profile, in samples of FMTs and disease-free tissues from the same animal. Results showed that and gene status are highly correlated (r=0.87, p<0.0001, n=25), with few tumor samples presenting amplification. Also, the majority of the FMTs showed overexpression coupled with overexpression (r=0.87, p<0.0001, n=27), being the -ICD and ECD transcripts highly correlated (r=0.97, p<0.0001, n=27). Significant associations were found between gene status or and RNA levels with several clinicopathological parameters. This work highlights the need of experimental designs for a precise evaluation of and gene status and its expression in FMTs, to improve their clinical management and to further validate them as a suitable model for comparative oncology studies.
在人类中，该基因扩增和过表达是浸润性乳腺癌的生物标志物及治疗靶点。此外，该基因畸变可预测蒽环类辅助化疗的反应。尽管猫乳腺肿瘤（FMTs）是比较肿瘤学中的良好模型，但关于该基因和另一基因的状态数据却很匮乏。在本研究中，首次将FMTs样本及同一动物的无病组织中该基因的细胞内（ICD）和细胞外（ECD）编码区的DNA状态及RNA水平与另一基因的状态和表达谱进行了比较。结果显示，该基因和另一基因的状态高度相关（r = 0.87，p < 0.0001，n = 25），仅有少数肿瘤样本出现扩增。此外，大多数FMTs显示该基因过表达并伴有另一基因过表达（r = 0.87，p < 0.0001，n = 27），其中该基因的 -ICD和ECD转录本高度相关（r = 0.97，p < 0.0001，n = 27）。该基因状态或另一基因与该基因的RNA水平和几个临床病理参数之间存在显著关联。这项工作强调了需要进行实验设计，以精确评估FMTs中该基因和另一基因的状态及其表达，从而改善其临床管理，并进一步验证它们作为比较肿瘤学研究的合适模型。
