Süss David, Iwersen Michael, Schweinzer Vanessa, Gusterer Erika, Kanz Peter, Krieger Stefanie, Pothmann Harald, Wagener Karen, Hoelker Michael, Tesfaye Dawit, Schellander Karl, Helmbrecht Ariane, Parys Claudia, Drillich Marc
Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health, University Clinic for Ruminants, University of Veterinary Medicine Vienna, Vienna, Austria.
Research Station Frankenforst, Faculty of Agriculture, University of Bonn, Königswinter, Germany.
Reprod Domest Anim. 2019 Sep;54(9):1265-1273. doi: 10.1111/rda.13509. Epub 2019 Aug 6.
There is evidence that supplementing methionine has positive effects on uterine environment, oocyte quality and embryo development in cattle. Thus, the objective of this study was to evaluate reproductive traits of cows supplemented with rumen-protected methionine (RPM) during early to mid-lactation in comparison with an untreated control group (CON). An additional focus was on the effect of puerperal diseases on reproductive performance parameters in RPM-supplemented group MET and in CON. A total of 1,709 multiparous Holstein-Friesian cows were enrolled in this field trial conducted on a commercial dairy farm in Slovakia. Cows were allocated at approximately 12 days post-partum (dpp) to either CON or MET, the latter supplemented with 25.0 g-27.2 g RPM per cow per day incorporated into the total mixed ration (TMR) until leaving the study pen at approximately 140 dpp. The amount of RPM was calculated based on individual feed ingredients analysis and adjusted during the study period when TMR changed. Cows were monitored during the post-partum period by vaginal examination (day 5 pp), measuring of beta-hydroxybutyrate in blood (3, 5, and 8 dpp) and by vaginal examination, uterine cytology and measuring of back fat thickness by ultrasound (all at 31 ± 3 dpp). Compared with CON, cows supplemented with RPM did not show better reproduction performance parameters (first service submission rate, days to first service, conception risk, days open 140). Results from binary logistic regression model for the risk of conception showed that metritis had a significant effect, but the supplementation of methionine had not. Results of Cox regression analysis for the odds of conception within 140 dpp revealed only metritis and clinical endometritis as significant factors. In conclusion, supplementation of RPM had no beneficial effect on reproductive performance in this study farm compared with an untreated control group.
有证据表明，补充蛋氨酸对奶牛的子宫环境、卵母细胞质量和胚胎发育具有积极影响。因此，本研究的目的是评估在泌乳早期至中期补充瘤胃保护性蛋氨酸（RPM）的奶牛与未处理的对照组（CON）相比的繁殖性状。另一个重点是产后疾病对补充RPM的MET组和CON组繁殖性能参数的影响。在斯洛伐克的一个商业奶牛场进行的这项田间试验中，共纳入了1709头经产荷斯坦-弗里生奶牛。奶牛在产后约12天（dpp）被分配到CON组或MET组，后者每头奶牛每天在全混合日粮（TMR）中添加25.0 g - 27.2 g RPM，直到在约140 dpp离开研究栏。RPM的添加量是根据个体饲料成分分析计算得出的，并在研究期间TMR发生变化时进行调整。在产后期间，通过阴道检查（产后第5天）、测量血液中的β-羟基丁酸（3、5和8 dpp）以及通过阴道检查、子宫细胞学检查和超声测量背部脂肪厚度（均在31±3 dpp）对奶牛进行监测。与CON组相比，补充RPM的奶牛在繁殖性能参数（首次配种提交率、首次配种天数、受孕风险、140天内的空怀天数）方面没有表现出更好的结果。二元逻辑回归模型关于受孕风险的结果表明，子宫炎有显著影响，但蛋氨酸补充没有。Cox回归分析关于140 dpp内受孕几率的结果显示，只有子宫炎和临床子宫内膜炎是显著因素。总之
