负载吲哚-3-乙酸和吲哚-3-丁酸的氧化锌纳米粒子的合成：对生根的影响。
Synthesis of indole-3-acetic acid and indole-3-butyric acid loaded zinc oxide nanoparticles: Effects on rhizogenesis.
机构信息
Ankara University, Chemical Engineering Department, Tandoğan, Ankara, 06100, Turkey.
Ankara University, Biotechnology Institute, Tandoğan, Ankara, 06100, Turkey.
出版信息
J Biotechnol. 2019 Sep 10;303:8-15. doi: 10.1016/j.jbiotec.2019.07.004. Epub 2019 Jul 10.
The aim of this work was to investigate the use of zinc oxide nanoparticles (nZnO) as nanocarriers for plant auxins indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) and determine the effects on rhizogenesis in micro cuttings of different Pyrus species. Auxin loaded nanoparticles (IAA-nZnO and IBA-nZnO) were characterized for particle size, morphology, thermal behavior and chemical structure. A high loading capacity was observed for both auxins (˜90%). Bioactivity assays were performed by using micro cuttings of Pyrus genotypes (Pyrus elaeagrifolia Pall and Pyrus communis L.) under aseptic conditions by dilute solution soaking method. In vitro rooting efficiency was increased at least two folds for the difficult-to-root wild pear (Pyrus elaeagrifolia Pallas) with IAA or IBA loaded ZnO nanoparticles. In this genotype, the highest rooting percentage was achieved for IBA-nZnO and IAA-nZnO at 400 mgL concentration as 50.0% and 41.7%, respectively. Thus, auxin loaded ZnO nanoparticles could be used as efficient nanocarriers in agricultural applications.
本工作旨在研究氧化锌纳米粒子（nZnO）作为植物生长素吲哚-3-乙酸（IAA）和吲哚-3-丁酸（IBA）的纳米载体的应用，并确定其对不同梨属植物微型插条生根的影响。负载有生长素的纳米粒子（IAA-nZnO 和 IBA-nZnO）的粒径、形态、热行为和化学结构进行了表征。两种生长素（~90%）的负载能力都很高。在无菌条件下，采用稀溶液浸泡法对梨基因型（Pyrus elaeagrifolia Pall 和 Pyrus communis L.）的微型插条进行生物活性测定。负载有 IAA 或 IBA 的 ZnO 纳米粒子至少将难以生根的野生梨（Pyrus elaeagrifolia Pallas）的生根效率提高了两倍。在这种基因型中，IBA-nZnO 和 IAA-nZnO 在 400 mg/L 浓度下的生根率最高，分别为 50.0%和 41.7%。因此，负载有生长素的 ZnO 纳米粒子可用作农业应用中的有效纳米载体。
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