Department of Tumour Pathology, Maria Sklodowska-Curie Institute- Oncology Center, Cracow Branch, Poland.
Department of Tumour Pathology, Maria Sklodowska-Curie Institute- Oncology Center, Cracow Branch, Poland.
Pathol Res Pract. 2019 Sep;215(9):152513. doi: 10.1016/j.prp.2019.152513. Epub 2019 Jun 28.
Some studies suggest that HPV infection may be important carcinogenic factor in development of some part of colorectal cancers. However, in the worldwide literature concerning this type of tumours, the great variability in HPV frequency is noticed. In Poland, the incidence of HPV infection in colorectal cancers was examined in five studies so far and their results are also conflicting. Therefore, the aim of the present study was to assess the HPV presence in the group of 120 patients with adenocarcinomas of rectum. HPV infection was assessed on the basis of DNA extracted from collected formalin fixed paraffin embedded tumour specimens. Viral presence was evaluated using two PCR based methods: nested PCR and quantitative PCR (qPCR) with primers specific for HPV16. All HPV positive samples were subjected to virus genotyping using AmoyDx Human papillomavirus (HPV) Genotyping Detection Kit and P16 immunostaining. Among 120 evaluated colorectal tumours, HPV DNA was detected in 2 cancers (1.67%) by nested PCR and in 2 (1.67%) tumours by qPCR, including 1 sample diagnosed as HPV positive on the basis of both PCR variants. Two HPV positive cancers had HPV16 infection and other one HPV18. All three tumours with positivity of HPV DNA were P16 negative. In south - central Poland, HPV infection in rectal cancers probably has not influence on rectal carcinogenesis.
一些研究表明，HPV 感染可能是某些结直肠癌发生的重要致癌因素。然而，在涉及此类肿瘤的全球文献中，HPV 频率的巨大变异性引起了关注。在波兰，迄今为止已有五项研究检查了 HPV 感染在结直肠癌中的发生率，其结果也存在争议。因此，本研究旨在评估 120 例直肠腺癌患者中 HPV 的存在情况。HPV 感染是基于从收集的福尔马林固定石蜡包埋肿瘤标本中提取的 DNA 进行评估的。使用两种基于 PCR 的方法：巢式 PCR 和针对 HPV16 的定量 PCR（qPCR）来评估病毒的存在。对所有 HPV 阳性样本进行病毒基因分型，使用厦门安普利 HPV 基因分型检测试剂盒和 P16 免疫组化。在评估的 120 例结直肠肿瘤中，通过巢式 PCR 在 2 例癌症（1.67%）中检测到 HPV DNA，在 2 例癌症（1.67%）中通过 qPCR 检测到 HPV DNA，包括 1 例基于两种 PCR 变体均诊断为 HPV 阳性的样本。两种 HPV 阳性癌症均为 HPV16 感染，另一种为 HPV18 感染。所有 3 例 HPV DNA 阳性肿瘤均为 P16 阴性。在波兰中南部，HPV 感染可能对直肠癌变没有影响。
