Biesaga Beata, Janecka-Widła Anna, Kołodziej-Rzepa Marta, Mucha-Małecka Anna, Słonina Dorota, Ziobro Marek, Wysocka Joanna, Adamczyk Agnieszka, Majchrzyk Kaja, Niemiec Joanna, Ambicka Aleksandra, Grela-Wojewoda Aleksandra, Harazin-Lechowska Agnieszka
Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, Wybrzeże Armii Krajowej 15, 44-101, Gliwice, Poland.
Department of Tumor Pathology, Maria Sklodowska-Curie National Research Institute of Oncology, Cracow Branch, Gliwice, Poland.
Infect Agent Cancer. 2021 Nov 27;16(1):67. doi: 10.1186/s13027-021-00405-z.
Some studies suggest that Human Papilloma Virus (HPV) infection is important factor in carcinogenesis of breast tumors. This study' objective was to analyze HPV prevalence in breast cancers of patients from south-central Poland.
The study was performed based on archival paraffin embebbed and formalin fixed blocks in the group of 383 patients with breast cancer. HPV prevalence and its genotype were assessed, respectively by: nested PCR (with two groups of primers: PGMY09/PGMY11 and GP5+/GP6+), quantitative PCR (qPCR). Tumors were classified as HPV positive in case of at least one positive result in nested PCR and positive results in genotyping procedure. For all HPV positive tissues P16 immunostaining was applied in order to confirm active viral infection.
In the group of 383 breast cancers, HPV positivity was found in 17 samples (4.4%) in nested PCR. All these samples were subjected to HPV genotyping. This analysis revealed presence of HPV type 16 into two tumors (0.5%). In these two cancers, P16 overexpression was reported.
In breast tumors of patients from south-central Poland in Poland, HPV positivity is demonstrated in very low percentage of cases.
一些研究表明，人乳头瘤病毒（HPV）感染是乳腺肿瘤发生的重要因素。本研究的目的是分析波兰中南部患者乳腺癌中HPV的流行情况。
本研究基于383例乳腺癌患者的存档石蜡包埋和福尔马林固定组织块进行。分别通过巢式PCR（使用两组引物：PGMY09/PGMY11和GP5+/GP6+）、定量PCR（qPCR）评估HPV的流行情况及其基因型。如果巢式PCR至少有一个阳性结果且基因分型程序结果为阳性，则将肿瘤分类为HPV阳性。对于所有HPV阳性组织，应用P16免疫染色以确认病毒的活跃感染。
在383例乳腺癌患者中，巢式PCR检测发现17个样本（4.4%）HPV呈阳性。所有这些样本均进行了HPV基因分型。该分析显示，有两个肿瘤（0.5%）存在16型HPV。在这两种癌症中，均报告有P16过表达。
在波兰中南部患者的乳腺肿瘤中，HPV阳性的病例比例非常低。
