工程化生物合成聚羟基烷酸酯（PHA）以实现多样性和降低成本。

Engineering biosynthesis of polyhydroxyalkanoates (PHA) for diversity and cost reduction.

机构信息

Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China; School of Life Sciences, Tsinghua University, Beijing, 100084, China.

Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China; School of Life Sciences, Tsinghua University, Beijing, 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China; Center for Nano- and Micro-Mechanics, Tsinghua University, Beijing, 100084, China; Dept of Chemical Engineering, Tsinghua University, Beijing, 100084, China.

出版信息

Metab Eng. 2020 Mar;58:82-93. doi: 10.1016/j.ymben.2019.07.004. Epub 2019 Jul 11.

DOI:10.1016/j.ymben.2019.07.004

PMID:31302223

Abstract

PHA, a family of natural biopolymers aiming to replace non-degradable plastics for short-term usages, has been developed to include various structures such as short-chain-length (scl) and medium-chain-length (mcl) monomers as well as their copolymers. However, PHA market has been grown slowly since 1980s due to limited variety with good mechanical properties and the high production cost. Here, we review most updated strategies or approaches including metabolic engineering, synthetic biology and morphology engineering on expanding PHA diversity, reducing production cost and enhancing PHA production. The extremophilic Halomonas spp. are taken as examples to show the feasibility and challenges to develop next generation industrial biotechnology (NGIB) for producing PHA more competitively.

摘要

PHA 是一类天然生物聚合物，旨在替代不可降解的塑料，用于短期使用。它的发展包括各种结构，如短链长度（scl）和中链长度（mcl）单体以及它们的共聚物。然而，自 20 世纪 80 年代以来，由于具有良好机械性能的品种有限和高生产成本，PHA 市场的发展一直较为缓慢。在这里，我们综述了最新的策略或方法，包括代谢工程、合成生物学和形态工程，以扩大 PHA 的多样性、降低生产成本和提高 PHA 的产量。嗜盐菌属 Halomonas spp. 被作为例子来说明开发下一代工业生物技术（NGIB）以更具竞争力地生产 PHA 的可行性和挑战。