MOE Key Laboratory for Industrial Biocatalysts, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Appl Microbiol Biotechnol. 2022 Nov;106(21):6977-6992. doi: 10.1007/s00253-022-12215-3. Epub 2022 Oct 7.
Halomonas spp. are the well-studied platform organisms or chassis for next-generation industrial biotechnology (NGIB) due to their contamination-resistant nature combined with their fast growth property. Several Halomonas spp. have been studied regarding their genomic information and molecular engineering approaches. Halomonas spp., especially Halomonas bluephagenesis, have been engineered to produce various biopolyesters such as polyhydroxyalkanoates (PHA), proteins including surfactants and enzymes, small molecular compounds including amino acids and derivates, as well as organic acids. This paper reviews all the progress reported in the last 10 years regarding this robust microbial cell factory. KEY POINTS: • Halomonas spp. are robust chassis for low-cost production of chemicals • Genomic information of some Halomonas spp. has been revealed • Molecular tools and approaches for Halomonas spp. have been developed • Halomonas spp. are becoming more and more important for biotechnology.
得益于耐污染性和快速生长的特点，盐单胞菌属（Halomonas spp.）已成为下一代工业生物技术（Next-generation industrial biotechnology，简称 NGIB）的研究热点平台生物或底盘生物。目前已经针对几种盐单胞菌属开展了基因组信息和分子工程方法的研究。已经对盐单胞菌属（特别是盐单胞菌蓝细菌）进行了工程改造，以生产各种生物聚合物，如聚羟基烷酸酯（Polyhydroxyalkanoates，简称 PHAs）、表面活性剂和酶等蛋白质、氨基酸及其衍生物等小分子化合物以及有机酸。本文综述了过去 10 年来在这个强大的微生物细胞工厂方面的所有进展。要点： • 盐单胞菌属是低成本生产化学品的稳健底盘生物 • 揭示了一些盐单胞菌属的基因组信息 • 开发了用于盐单胞菌属的分子工具和方法 • 盐单胞菌属在生物技术中的重要性日益凸显。
