Sarciron M E, Azzar G, Got R, Petavy A F
Département de Parasitologie et Pathologie Exotique, Université Claude Bernard Lyon 1 (UER Faculté de Pharmacie), Villeurbanne, France.
Exp Parasitol. 1988 Jun;66(1):1-6. doi: 10.1016/0014-4894(88)90043-4.
A comparative study of the kinetic parameters of glycogen synthase was performed on Echinococcus multilocularis metacestodes and on the livers of infected and control host (Meriones unguiculatus). The enzyme of the parasite was found to be different from the enzyme of infected host liver. The apparent Km for UDP-glucose is 100 microM for the parasite and 400 microM for the host liver. The apparent Km for glucose 6-phosphate is 4 mM for the parasite and 2 mM for the host liver. The apparent Km for glycogen is 16 mg/ml for the parasite and 125 mg/ml for the host liver. The influence of glucose 6-phosphate and exogenous glycogen on the activity of glycogen synthase differs between the metacestode and the host liver. The enzyme of the metacestodes apparently does not need exogenous glycogen to work, contrary to the case for the liver host enzyme. The glycogen synthase of the parasite seems to be present in forms I and D, whereas the enzyme of the host liver appears in form I and that of the control liver in form D.
对多房棘球绦虫的原头蚴以及感染和对照宿主（长爪沙鼠）的肝脏进行了糖原合酶动力学参数的比较研究。发现寄生虫的酶与感染宿主肝脏的酶不同。寄生虫中UDP-葡萄糖的表观Km为100微摩尔，宿主肝脏中为400微摩尔。寄生虫中6-磷酸葡萄糖的表观Km为4毫摩尔，宿主肝脏中为2毫摩尔。寄生虫中糖原的表观Km为16毫克/毫升，宿主肝脏中为125毫克/毫升。原头蚴和宿主肝脏中6-磷酸葡萄糖和外源性糖原对糖原合酶活性的影响不同。与肝脏宿主酶的情况相反，原头蚴的酶显然不需要外源性糖原就能发挥作用。寄生虫的糖原合酶似乎以I型和D型存在，而宿主肝脏的酶以I型出现，对照肝脏的酶以D型出现。
