Bioanalytical Development, Bruker Daltonik GmbH , Bremen , Germany.
Institute of Clinical Microbiology, University of Szeged , Szeged , Hungary.
Expert Rev Mol Diagn. 2019 Aug;19(8):667-682. doi: 10.1080/14737159.2019.1643238. Epub 2019 Jul 25.
: Ten years after its introduction into clinical microbiology, MALDI-TOF mass spectrometry has become the standard routine identification tool for bacteria in most laboratories. The technology has accelerated analyses and improved the quality of results. The greatest significance has been observed for bacteria that were challenging to be identified by traditional methods. : We searched in existing literature (Pubmed) for reports how MALDI-TOF MS has contributed to identification of rare and unknown bacteria from different groups. We describe how this has improved the diagnostics in different groups of bacteria. Reference patterns for strains which yet cannot be assigned to a known species even enable the search for related bacteria in studies as well as in routine diagnostics. MALDI-TOF MS can help to discover and investigate new species and their clinical relevance. It is a powerful tool in the elucidation of the bacterial composition of complex microbiota in culturomics studies. : MALDI-TOF MS has improved the diagnosis of bacterial infections. It also enables knowledge generation for prospective diagnostics. The term 'hard-to-identify' might only be rarely attributed to bacteria in the future. Novel applications are being developed, e.g. subspecies differentiation, typing, and antibiotic resistance testing which may further contribute to improved microbial diagnostics.
: 自 MALDI-TOF 质谱分析技术引入临床微生物学领域十年以来，它已成为大多数实验室鉴定细菌的标准常规工具。该技术加速了分析并提高了结果的质量。对于那些用传统方法难以鉴定的细菌，其意义尤为重大。 : 我们在现有文献（Pubmed）中搜索了关于 MALDI-TOF MS 如何有助于鉴定来自不同群组的罕见和未知细菌的报告。我们描述了它如何改善了不同群组细菌的诊断。对于那些尚未能分配到已知种的菌株，参考图谱甚至可以在研究和常规诊断中搜索相关细菌。MALDI-TOF MS 有助于发现和研究新的细菌及其临床相关性。它是阐明培养组学研究中复杂微生物群落细菌组成的有力工具。 : MALDI-TOF MS 提高了细菌感染的诊断水平。它还为前瞻性诊断提供了知识基础。将来，“难以鉴定”这个词可能很少用于细菌。正在开发新的应用，例如亚种分化、分型和抗生素耐药性检测，这可能进一步有助于提高微生物诊断水平。
