Masuda R, Yoshida M C, Sasaki M, Dempo K, Mori M
Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo.
Jpn J Cancer Res. 1988 Feb;79(2):250-4. doi: 10.1111/j.1349-7006.1988.tb01584.x.
A serially transplantable rat hepatocellular carcinoma (HCC) line was established. The primary spontaneous HCC which developed in a 506-day-old male hereditary hepatitis LEC rat was inoculated into young LEC rats. Only this HCC of 18 primary HCCs was successful in serial transplantation. The established cell line was histologically identical to the primary HCC showing a well-differentiated type with a trabecular structure of tumorous hepatocytes. The characteristic of albumin production was maintained. Chromosome analysis revealed rather widely dispersed polyploid chromosome numbers with a modal value at 96. Every metaphase contained two to five unusually large marker chromosomes.
建立了一种可连续传代移植的大鼠肝细胞癌（HCC）细胞系。将一只506日龄雄性遗传性肝炎LEC大鼠原发的自发性HCC接种到年轻的LEC大鼠体内。18个原发性HCC中只有这一个HCC成功进行了连续移植。所建立的细胞系在组织学上与原发性HCC相同，表现为高分化型，具有肿瘤性肝细胞的小梁结构。白蛋白产生的特性得以保持。染色体分析显示多倍体染色体数分布较为广泛，众数为96。每个中期相中含有两到五条异常大的标记染色体。
