Omata M, Uchiumi K, Ito Y, Yokosuka O, Mori J, Terao K, Wei-Fa Y, O'Connell A P, London W T, Okuda K
Gastroenterology. 1983 Aug;85(2):260-7.
The presence of duck hepatitis B virus in serum was studied in 61 ducks (24 from Chi-tung county, China, 20 from Changchun, China, and 17 from Chiba, Japan) with relation to liver disease. None of the 37 ducks from Chiba and Changchun was positive for duck hepatitis B virus as assayed by electron microscopy, endogenous deoxyribonucleic acid-polymerase activity, and hybridization with duck hepatitis B virus deoxyribonucleic acid. No liver disease was seen in these ducks. In contrast, viruslike particles were present in the serum of 12 of 24 (50%) ducks from Chi-tung, China. The presence of duck hepatitis B virus in serum was indicated by the hybridization spot test and deoxyribonucleic acid-polymerase activities. A variety of liver diseases including chronic hepatitis and cirrhosis were seen in the livers of a majority of the ducks from Chi-tung. One duck hepatitis B virus-positive duck had multicentric hepatocellular carcinoma with underlying cirrhosis. Comparison of serum duck hepatitis B virus markers and liver disease in the affected flock revealed a tendency for seronegative ducks to have advanced liver diseases. Duck hepatitis B virus infection may be used as an experimental model to test various hypotheses concerning the pathogenesis of hepatitis B virus-associated liver disease in humans.
对61只鸭（24只来自中国启东县，20只来自中国长春，17只来自日本千叶县）血清中的鸭乙型肝炎病毒与肝脏疾病的关系进行了研究。通过电子显微镜、内源性脱氧核糖核酸聚合酶活性以及与鸭乙型肝炎病毒脱氧核糖核酸杂交检测，千叶县和长春的37只鸭均未检测出鸭乙型肝炎病毒阳性。这些鸭未出现肝脏疾病。相比之下，中国启东的24只鸭中有12只（50%）血清中存在病毒样颗粒。杂交斑点试验和脱氧核糖核酸聚合酶活性表明血清中存在鸭乙型肝炎病毒。启东的大多数鸭肝脏出现了包括慢性肝炎和肝硬化在内的多种肝脏疾病。一只鸭乙型肝炎病毒阳性鸭患有多中心肝细胞癌并伴有潜在肝硬化。对受感染鸭群血清鸭乙型肝炎病毒标志物与肝脏疾病的比较显示，血清阴性鸭有患晚期肝脏疾病的倾向。鸭乙型肝炎病毒感染可作为一种实验模型，用于检验有关人类乙型肝炎病毒相关肝脏疾病发病机制的各种假说。
