固定化脂肪酶B催化合成季戊四醇单蓖麻醇酸酯（PEMR）
Production of Pentaerythritol Monoricinoleate (PEMR) by immobilized lipase B.
作者信息
Yadav Manish G, Vadgama Rajeshkumar N, Kavadia Monali R, Odaneth Annamma Anil, Lali Arvind M
机构信息
DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology (ICT, Formerly UDCT/UICT), University under Section 3 of UGC Act - 1956, Elite Status & Centre of Excellence - Government of Maharashtra, TEQIP Phase II funded, Nathalal Parekh Marg, Matunga, Mumbai, 400019, India.
出版信息
Biotechnol Rep (Amst). 2019 Jun 16;23:e00353. doi: 10.1016/j.btre.2019.e00353. eCollection 2019 Sep.
In the present study, green synthesis of pentaerythritol monoricinoleate (PEMR) was carried out using lipase B immobilized on hydrophobic adsorbent via interfacial activation. Various reaction parameters such as reaction time, organic solvent, molar ratio, the enzyme load, and presence of molecular sieves on pentaerythritol (PE) ester synthesis were systematically studied to yield selective monoester of PE. The strategies (smart use of substrate molar ratio and polar organic solvent) were employed to suppress dimerization of ricinoleic acid (RA) to avoid by-product formation and hence to obtain superior mono-ester yield. Under optimized conditions substrate molar ratio of 4 (PE):1 (RA) with 2% enzyme load and 200 g/L molecular sieves in the presence of -butanol, 93% substrate molar conversion in 24 h reaction time was obtained. The synthesized PEMR was also characterized using FT-IR and Mass spectroscopy. To the best of our knowledge, this is the first report describing the enzymatic synthesis of PEMR.
在本研究中，通过界面活化法将脂肪酶B固定在疏水吸附剂上，进行了季戊四醇单蓖麻油酸酯（PEMR）的绿色合成。系统研究了各种反应参数，如反应时间、有机溶剂、摩尔比、酶负载量以及分子筛的存在对季戊四醇（PE）酯合成的影响，以获得PE的选择性单酯。采用了一些策略（巧妙利用底物摩尔比和极性有机溶剂）来抑制蓖麻油酸（RA）的二聚化，避免副产物的形成，从而获得较高的单酯产率。在优化条件下，以丁醇为溶剂，底物摩尔比为4（PE）:1（RA），酶负载量为2%，分子筛为200 g/L，在24小时反应时间内底物摩尔转化率达到93%。还使用傅里叶变换红外光谱（FT-IR）和质谱对合成的PEMR进行了表征。据我们所知，这是第一篇描述PEMR酶促合成的报告。
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