Department of Oncology (Section 3), Gaozhou People's Hospital, Gaozhou, Guangdong, PR China.
Department of internal medicine, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, PR China.
Epigenomics. 2019 Aug;11(11):1335-1352. doi: 10.2217/epi-2019-0121. Epub 2019 Jul 15.
To study the role of circRNA (circ_0001730) in glioblastoma. The interaction between circ_0001730 and miR-326 was confirmed by FISH, RNA pull down, RNA-binding protein immunoprecipitation and luciferase reporter assays. Cell proliferation and growth were determined by MTT, EdU and colony formation assays. Cell migration was assessed by the Boyden assay. The levels of circ_0001730 were elevated in glioblastoma cell lines and tissues. circ_0001730 downregulation suppressed migration and proliferation in glioblastoma cells. SP1 bounds to the promoter of circ_0001730 host gene EPHB4 thereby increasing the expression of circ_0001730. circ_0001730 activated the Wnt/β-catenin pathway via the miR-326/Wnt7B axis. circ_000173 promoted growth and invasion in glioblastoma cells via the miR-326/Wnt7B axis.
研究环状 RNA（circ_0001730）在神经胶质瘤中的作用。通过 FISH、RNA 下拉、RNA 结合蛋白免疫沉淀和荧光素酶报告基因检测证实了 circ_0001730 与 miR-326 之间的相互作用。通过 MTT、EdU 和集落形成实验测定细胞增殖和生长。通过 Boyden 测定评估细胞迁移。circ_0001730 在神经胶质瘤细胞系和组织中上调。circ_0001730 下调抑制神经胶质瘤细胞的迁移和增殖。SP1 结合到 circ_0001730 宿主基因 EPHB4 的启动子上，从而增加 circ_0001730 的表达。circ_0001730 通过 miR-326/Wnt7B 轴激活 Wnt/β-catenin 通路。circ_000173 通过 miR-326/Wnt7B 轴促进神经胶质瘤细胞的生长和侵袭。
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