Zhai Zhen, Yu Panxi, Huang Minlu, Jin Xiaolei, Yang Xiaonan, Qi Zuoliang
Department No. 16 of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
J Craniofac Surg. 2019 Nov-Dec;30(8):2332-2336. doi: 10.1097/SCS.0000000000005745.
Fat acquisition, processing, and grafting techniques are considered to be the main factors affecting the volume retention rate of fat grafting. Shuffling fat (SF) is obtained by refining macrofat (MF), both of which have been applied in autologous fat grafting. However, few studies have focused on comparison effects of platelet-rich fibrin (PRF) on MF and SF grafting. Grafts were prepared by mixing MF and SF with PRF or normal saline at a ratio of 5:1, and grafted into symmetric recipient sites of a mouse's buttock. Grafts were harvested and analyzed at 4, 8, and 12 weeks. Groups containing PRF showed high volume retention rates and microcosmic evaluation scores at each time point compared with the control groups. Although without improved volume retention rate, group of SF containing PRF showed superior microcosmic evaluations compared with group of MF containing PRF. The results demonstrated that both the efficacy of MF and SF grafting can be enhanced by PRF, with even better quality of grafts for the SF grafting.
脂肪获取、处理及移植技术被认为是影响脂肪移植体积保留率的主要因素。通过提炼粗大脂肪（MF）获得搅碎脂肪（SF），二者均已应用于自体脂肪移植。然而，很少有研究关注富血小板纤维蛋白（PRF）对MF和SF移植的比较效果。将MF和SF按5:1的比例与PRF或生理盐水混合制备移植物，并移植到小鼠臀部的对称受体部位。在4周、8周和12周时收获并分析移植物。与对照组相比，含PRF的组在每个时间点均显示出较高的体积保留率和微观评估分数。虽然含PRF的SF组未提高体积保留率，但其微观评估优于含PRF的MF组。结果表明，PRF可提高MF和SF移植的效果，且SF移植的移植物质量更好。
