Shaw J H, Hayes F, Brooks G F, Falkow S
Dept. of Medical Microbiology, Stanford University, CA.
Antonie Van Leeuwenhoek. 1987;53(6):485-91. doi: 10.1007/BF00415507.
Neisseria gonorrhoeae invasion of the human endometrial cell line HecIB was monitored by electron microscopy. Within six hours postinfection, the gonococci have attached to the surface of some HecIB cells and are embraced by microvilli. Gonococci subsequently enter the HecIB cells in membrane bound vesicles but by eight hours, gonococci can be seen free in the cytoplasm. At twelve hours post-infection some HecIB cells are observed containing hundreds of internalized bacteria. At twenty-four hours gonococci appear in large clusters embedded in a matrix of cellular debris, which are possibly the remains of lysed infected cells. In contrast, N. lactamica is adherent to the monolayer but noninvasive.
通过电子显微镜监测淋病奈瑟菌对人子宫内膜细胞系HecIB的侵袭。感染后6小时内，淋球菌已附着在一些HecIB细胞表面，并被微绒毛包围。随后，淋球菌通过膜结合囊泡进入HecIB细胞，但在8小时时，可在细胞质中看到游离的淋球菌。感染后12小时，观察到一些HecIB细胞含有数百个内化细菌。在24小时时，淋球菌以大簇形式出现在嵌入细胞碎片基质中的区域，这些碎片可能是被裂解的感染细胞的残余物。相比之下，乳酸奈瑟菌附着于单层细胞但无侵袭性。
