Hua Rui, Zhou Lu, Zhang Haiwen, Yang Hui, Peng Wenchuan, Wu Kebang
Key Laboratory of Tropical Animal Breeding and Epidemic Disease Research of Hainan Province, Hainan University, Hainan 570228, People's Republic of China.
Laboratory of Tropical Animal Breeding, Reproduction and Nutrition, Hainan University, Hainan 570228, People's Republic of China.
J Reprod Dev. 2019 Oct 23;65(5):413-421. doi: 10.1262/jrd.2018-105. Epub 2019 Jul 14.
The Hainan black goat is a high-quality local goat breed in Hainan Province of China. It is resistant to high temperatures, humidity, and disease. Although the meat of this breed is tender and delicious, its reproductive performance and milk yield are low. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) technology was used to analyze the differentially expressed proteins in the serum of female Hainan black goats during the reproductive cycle (empty pregnant, estrus, gestation, and lactation). The pathway enrichment analysis results showed that most of the differentially expressed proteins between each period belonged to the complement and coagulation cascades. Analysis of the differential protein expression and function revealed seven proteins that were directly associated with reproduction, namely pre-SAA21, ANTXR2, vWF, SFRP3, β4GalT1, pre-IGFBP2 and Ran. This study revealed the changing patterns of differentially expressed proteins in the reproductive cycle of the Hainan black goat. pre-SAA21, ANTXR2, vWF, SFRP3, β4GalT1, pre-IGFBP2, and Ran were identified as candidate proteins for mediating the physiological state of Hainan black goats and regulating their fertility. This study elucidated the changes in expression levels of differentially expressed proteins during the reproductive cycle of Hainan black goats and also provides details about its breeding pattern.
海南黑山羊是中国海南省的一个优质地方山羊品种。它耐高温、耐潮湿且抗病。尽管该品种的肉鲜嫩美味，但其繁殖性能和产奶量较低。在本研究中，采用相对和绝对定量同位素标记（iTRAQ）技术分析了海南黑山羊母羊在生殖周期（空怀期、发情期、妊娠期和哺乳期）血清中差异表达的蛋白质。通路富集分析结果表明，各时期之间的大多数差异表达蛋白质属于补体和凝血级联反应。对差异蛋白质表达和功能的分析揭示了七种与繁殖直接相关的蛋白质，即前SAA21、炭疽毒素受体2（ANTXR2）、血管性血友病因子（vWF）、分泌型卷曲相关蛋白3（SFRP3）、β1,4-半乳糖基转移酶1（β4GalT1）、前胰岛素样生长因子结合蛋白2（pre-IGFBP2）和Ran。本研究揭示了海南黑山羊生殖周期中差异表达蛋白质的变化模式。前SAA21、ANTXR2、vWF、SFRP3、β4GalT1、pre-IGFBP2和Ran被确定为介导海南黑山羊生理状态和调节其繁殖力的候选蛋白质。本研究阐明了海南黑山羊生殖周期中差异表达蛋白质表达水平的变化，也提供了其繁殖模式的详细信息。
