Wang Zhongchuan, Li Rujia, He Yongshan, Huang Shiyong
Department of Colorectal Surgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University, School of Medicine, No. 1665 Kongjiang Road, Shanghai, 200092 China.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRPs family that modulates the Wnt signal transduction pathway. Recent studies have showed down-regulation of SFRP1 expression in colorectal cancer (CRC). We aimed to evaluate the effect of SFRP1 on the proliferation, migration, invasion and apoptosis of CRC cells in vitro.
We used real-time fluorescence quantification (RT-PCR) and Western blotting to detect SFRP1 expression in CRC, pericarcinomatous tissues and CRC cell lines. We assessed the influence of overexpression and knockdown of SFRP1 on CRC cell proliferation, migration, invasion, and apoptosis, Western blotting was used to evaluate protein levels of Wnt, β-catenin, and apoptosis-related proteins.
The expression of SFRP1 was significantly decreased in CRC tissues. Among the six CRC cell lines (sw-480, sw1116, caco-2, ht-29, colo-205, and hct-116), RT-PCR revealed that sw1116 cells had the lowest expression of SFRP1, while caco-2 cells had the highest SFRP1 expression. SFRP1 overexpression in sw1116 cells significantly suppressed cell proliferation while SFRP1 knockdown in caco-2 cells significantly increase the cell proliferation. In addition, overexpression of SFRP1 in sw1116 cells remarkedly suppressed cell migration and invasion, whereas knockdown of SFRP1 in caco-2 cells resulted in significant enhancement of migration and invasion. Furthermore, SFRP1 overexpression in sw1116 cells promoted cell apoptosis. Western blotting showed that SFRP1 overexpression significantly decreased the protein levels of Wnt, β-catenin and apoptosis-related proteins, including MMP2, MMP9, Twist, CDK1, TGF, and Bcl2.
Our results demonstrate that SFRP1 suppresses cell proliferation, migration and invasion, and promotes apoptosis in CRC cells.
分泌型卷曲相关蛋白1（SFRP1）是SFRPs家族的成员，可调节Wnt信号转导通路。最近的研究表明，SFRP1在结直肠癌（CRC）中的表达下调。我们旨在评估SFRP1对CRC细胞体外增殖、迁移、侵袭和凋亡的影响。
我们使用实时荧光定量（RT-PCR）和蛋白质印迹法检测CRC、癌旁组织和CRC细胞系中SFRP1的表达。我们评估了SFRP1过表达和敲低对CRC细胞增殖、迁移、侵袭和凋亡的影响，蛋白质印迹法用于评估Wnt、β-连环蛋白和凋亡相关蛋白的水平。
SFRP1在CRC组织中的表达显著降低。在六种CRC细胞系（sw-480、sw1116、caco-2、ht-29、colo-205和hct-116）中，RT-PCR显示sw1116细胞中SFRP1的表达最低，而caco-2细胞中SFRP1的表达最高。sw1116细胞中SFRP1过表达显著抑制细胞增殖，而caco-2细胞中SFRP1敲低显著增加细胞增殖。此外，sw1116细胞中SFRP1过表达显著抑制细胞迁移和侵袭，而caco-2细胞中SFRP1敲低导致迁移和侵袭显著增强。此外，sw1116细胞中SFRP1过表达促进细胞凋亡。蛋白质印迹法显示，SFRP1过表达显著降低Wnt、β-连环蛋白和凋亡相关蛋白的水平，包括MMP2、MMP9、Twist、CDK1、TGF和Bcl2。
我们的结果表明，SFRP1抑制CRC细胞的增殖、迁移和侵袭，并促进其凋亡。
