通过红外光谱和血清学方法对奇异变形杆菌脂多糖样品进行表征

Characterization of Proteus mirabilis Lipopolysaccharide Samples by Infrared Spectroscopy and Serological Methods.

作者信息

Durlik Katarzyna, Czerwonka Grzegorz, Żarnowiec Paulina, Kaca Wiesław

机构信息

Department of Microbiology, Institute of Biology, Jan Kochanowski University in Kielce, Kielce, Poland.

出版信息

Methods Mol Biol. 2019;2021:217-230. doi: 10.1007/978-1-4939-9601-8_19.

DOI:10.1007/978-1-4939-9601-8_19

PMID:31309508

Abstract

Methods of lipopolysaccharide extraction, purification, and sample validation are presented. Based on serological reaction in ELISA, immunoblotting, and infrared spectra, identities of two LPS preparations from smooth P. mirabilis (O18) PrK 34/57 are presented.

摘要

介绍了脂多糖的提取、纯化及样品验证方法。基于酶联免疫吸附测定（ELISA）、免疫印迹和红外光谱中的血清学反应，给出了来自奇异变形杆菌（O18）PrK 34/57光滑型的两种脂多糖制剂的鉴定结果。