Kaca Wiesław, Gleńska-Olender Joanna, Konieczna Iwona, Gawęda Józef, Sęk Sławomir
Department of Microbiology, Institute of Biology, Jan Kochanowski University in Kielce, Kielce, Poland.
Regional Science and Technology Center, Świętokrzyski Biobank, Chęciny, Poland.
Methods Mol Biol. 2019;2021:273-283. doi: 10.1007/978-1-4939-9601-8_23.
Atomic force microscopy (AFM) is being increasingly used to directly measure protein interactions in nearly physiological environments. Here, protocols for atomic force microscopy (AFM) for visualization of antigen-antibody complexes are presented. The technique is used to demonstrate complexes formed by rheumatoid arthritis patient antibodies with lipopolysaccharide (LPS) isolated from P. mirabilis (O3) strain S1959 and a synthetic antigen (LPS epitope of 6 N-alpha-(D-galacturonoyl)-L-lysine residues).
原子力显微镜（AFM）越来越多地用于在近乎生理的环境中直接测量蛋白质相互作用。本文介绍了用于可视化抗原-抗体复合物的原子力显微镜（AFM）实验方案。该技术用于展示类风湿性关节炎患者抗体与从奇异变形杆菌（O3）菌株S1959分离的脂多糖（LPS）以及一种合成抗原（6个N-α-（D-半乳糖醛酰基）-L-赖氨酸残基的LPS表位）形成的复合物。
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