Edgar L G, McGhee J D
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Cell. 1988 May 20;53(4):589-99. doi: 10.1016/0092-8674(88)90575-2.
DNA synthesis in each cell lineage of the early C. elegans embryo was measured using microspectrofluorimetry. Aphidicolin was shown to inhibit DNA synthesis almost instantly and completely. Aphidicolin was then used to investigate how DNA synthesis controls expression of two biochemical markers that appear at different times during gut development: gut granules and a carboxylesterase. We show that marker expression is controlled neither by reaching the normal DNA: cytoplasm ratio, by counting the normal number of rounds of DNA synthesis, nor by a simple lengthening of the cell cycle. Instead, expression of both gut markers requires a short period of DNA synthesis in the first cell cycle after the gut has been clonally established.
利用显微分光荧光测定法测量了秀丽隐杆线虫早期胚胎各细胞谱系中的DNA合成。结果显示，阿非迪霉素几乎能立即完全抑制DNA合成。随后，利用阿非迪霉素研究了DNA合成如何控制肠道发育过程中不同时间出现的两种生化标志物的表达：肠道颗粒和一种羧酸酯酶。我们发现，标志物的表达既不是由达到正常的DNA：细胞质比例、计算正常的DNA合成轮数来控制，也不是由细胞周期的简单延长来控制。相反，两种肠道标志物的表达都需要在肠道克隆建立后的第一个细胞周期中进行短时间的DNA合成。
