sp. nov., producer of semduramicin, a polyether ionophore.

The taxonomic position of '' LMG 30035, a semduramicin-producing mutant of strain ATCC 53666, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach. This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores. Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035 was found to be closely related to the type strains of ATCC 49459 (98.88 %) and JCM 7474 (98.82 %) (pairwise similarity values in parentheses). Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035 represents a novel species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named species). Analysis of the cell wall revealed the presence of -diaminopimelic acid in the peptidoglycan. The whole-cell sugars were glucose, madurose, galactose, ribose and rhamnose. The major polar lipids included phosphatidylinositol and diphosphatidylglycerol. The predominant menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were C, 10-methyl C, C  and C. The DNA G+C content of its genome was 72.5 mol%. In summary, these characteristics distinguish strain LMG 30035 from validly named species of the genus , and therefore, we propose to classify this strain formally as the novel species sp. nov. with LMG 30035 (=CECT 9808,=ATCC 53664) as the type strain.