Description of the bacterial RNA polymerase inhibitor GE23077-producer sp. NRRL B-65521 as sp. nov.

The filamentous actinomycete that produces the antibiotic GE23077 was isolated by the Lepetit Research Group from a soil sample collected in Thailand, and it was classified as a member of the genus on the basis of its morphology and cell-wall composition. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain formed a distinct monophyletic line within the genus and it was most closely related to DSM 45347 (99.31 % similarity) and DSM 44485 (98.94 %). The GE23077-producing strain formed an extensively branched, non-fragmented vegetative mycelium; no pseudosporangia were formed and the arthrospores were organized in slightly twisted chains. The cell wall contained -2,6-diaminopimelic acid and the diagnostic sugar was madurose. The predominant menaquinone was MK-9(H), with minor amounts of MK-9(H) and MK-9(H). The diagnostic phospholipids were phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acids were C and tuberculostearic acid (10-methyloctadecanoic acid), followed by minor amounts of Cω9, Cω7 and 10-methylheptadecanoic acid. The genomic DNA G+C content was 71.77 mol%. Significant differences in the morphological, chemotaxonomic and biochemical data, and the low DNA-DNA relatedness between the GE23077-producing strain and closely related type strains clearly demonstrate that it represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NRRL B-65521(=LMG 31258=DSM 109019).