Postgraduate Department of Endodontics, Universidad Central de Venezuela, Caracas, Venezuela.
Centro de Investigaciones Odontológicas, Pontificia Universidad Javeriana, Bogotá, Colombia.
Int Endod J. 2019 Dec;52(12):1716-1722. doi: 10.1111/iej.13188. Epub 2019 Aug 5.
To quantify the expression of angiogenic growth factors (ANG2, VEGFA, TGFß1) and their corresponding receptors (VEGFR1, VGFR2, NRP1 and TGFßR1) in human dental pulps from extracted third molars with complete and incomplete root development.
Fifty-six dental pulp samples obtained from freshly extracted human third molars were divided equally into two groups according to their stage of root development; 28 third molars with complete root development and 28 third molars with incomplete root development. All samples were processed and total RNA was extracted, cDNA was then synthetized for each sample and the target genes expression profiles for ANG2, VEGFA, VEGFR1, VEGFR2, NRP1, TGFß1 and TGFßR1 were obtained by RT2-PCR. The data was analysed with a Student's t-test to compare the replicate ∆∆C values for each gene.
Teeth with incomplete root development were associated with a significantly greater gene expression of TGFβR1 (P = 0.03), whereas in teeth with complete root development the genes that had significantly greater expression were VEGFA (P = 0.04).
The angiogenic growth factors (ANG2, VEGFA, TGFβ1) and their receptors (NRP1, VEGFR1, VEGFR2 and TGFßR1) were expressed in pulps of teeth with complete and incomplete root development measured by RT2-PCR, with TGFBR1 genes being significantly different in teeth with incomplete root development and VEGFA genes in teeth with complete root development.
定量检测完全和不完全发育的人类第三磨牙牙髓中血管生成生长因子（ANG2、VEGFA、TGFβ1）及其相应受体（VEGFR1、VEGFR2、NRP1 和 TGFβR1）的表达。
从新鲜拔出的第三磨牙中获得 56 个牙髓样本，根据根发育阶段将它们平均分为两组；28 个具有完全根发育的第三磨牙和 28 个具有不完全根发育的第三磨牙。所有样本均经过处理并提取总 RNA，然后为每个样本合成 cDNA，并通过 RT2-PCR 获得 ANG2、VEGFA、VEGFR1、VEGFR2、NRP1、TGFβ1 和 TGFβR1 的目标基因表达谱。使用学生 t 检验分析数据，以比较每个基因的重复 ∆∆C 值。
不完全根发育的牙齿与 TGFβR1 基因表达显著增加相关（P = 0.03），而在完全根发育的牙齿中，VEGFA 基因表达显著增加（P = 0.04）。
通过 RT2-PCR 测量，完全和不完全根发育的牙齿牙髓中表达了血管生成生长因子（ANG2、VEGFA、TGFβ1）及其受体（NRP1、VEGFR1、VEGFR2 和 TGFβR1），在不完全根发育的牙齿中 TGFBR1 基因和在完全根发育的牙齿中 VEGFA 基因的表达有显著差异。
