Department of Endodontics, Universidad Central de Venezuela, Caracas, Venezuela.
Centro de Investigaciones Odontológicas, Faculty of Dentistry, Pontificia Universidad Javeriana, Bogotá.
Eur Endod J. 2021 Dec;6(3):259-263. doi: 10.14744/eej.2021.86580.
Vascular endothelial growth factor A (VEGFA) and its receptors are essential proteins for the angiogenic activity of dental pulp. Angiogenesis fundamentally provides oxygen and nutrients to cells for root formation and defence mechanisms. The angiogenic potential of dental pulp should be understood and considered for the conservative and regenerative endodontics. The purpose of this research was to measure the VEGFA expression and its receptors such as vascular endothelial growth factor receptors 1, -2 (VEGFR1, VEGFR2) and Neuropilin 1 (NRP1) in human dental pulp from molars with immature and mature apexes.
VEGFA system mRNAs expressions were assessed in dental pulp obtained from freshly extracted human third molars divided into immature (n=8) and mature (n=8) apexes. RNAs were extracted from the samples. Each sample's cDNA was synthetized and the target genes VEGFA, VEGFR1, VEGFR2, NRP1 expression profiles obtained by RT2-PCR. Analysis was based on the Student's t-test comparing the replicate 2-ΔCt values for each gene. P values of <0.05 were considered significant.
In teeth with mature apexes, VEGFA (P=0.0002), NRP1 (P=0.0001), VEGFR1 (P=0.0057) and VEGFR2 (P=0.018259) significantly increased statistically with respect to the immature apexes group.
Within the limitation of the present investigation, it can be concluded that the angiogenic process seems to be a physiological process in the dental pulp due to the studied angiogenic growth factor are expressed in both immature and mature dental pulps. VEGFA and its receptors are expressed significantly higher in mature apex teeth than immature apex teeth.
血管内皮生长因子 A（VEGFA）及其受体是牙髓血管生成活性所必需的蛋白。血管生成从根本上为细胞提供氧气和营养物质，以促进根尖的形成和防御机制。了解牙髓的血管生成潜力对于保守和再生性牙髓治疗至关重要。本研究旨在测量人磨牙未成熟和成熟根尖牙髓中血管内皮生长因子 A（VEGFA）及其受体，如血管内皮生长因子受体 1、-2（VEGFR1、VEGFR2）和神经纤毛蛋白 1（NRP1）的表达。
从新鲜提取的人第三磨牙中获取牙髓样本，分为未成熟（n=8）和成熟（n=8）根尖，并评估 VEGFA 系统 mRNA 的表达。从样本中提取 RNA。合成每个样本的 cDNA，并通过 RT2-PCR 获得目标基因 VEGFA、VEGFR1、VEGFR2、NRP1 的表达谱。分析基于比较每个基因的重复 2-ΔCt 值的 Student's t 检验。P 值<0.05 被认为具有统计学意义。
在具有成熟根尖的牙齿中，VEGFA（P=0.0002）、NRP1（P=0.0001）、VEGFR1（P=0.0057）和 VEGFR2（P=0.018259）的表达显著增加。
在本研究的限制范围内，可以得出结论，由于研究的血管生成生长因子在未成熟和成熟牙髓中均有表达，因此血管生成过程似乎是牙髓的一种生理过程。VEGFA 及其受体在成熟根尖牙齿中的表达明显高于未成熟根尖牙齿。
