Centro de Investigaciones Odontologicas, Pontificia Universidad Javeriana, Bogota, Colombia.
Endodontics Department, Universidad Cooperativa de Colombia, Pasto, Colombia.
BMC Oral Health. 2020 Nov 12;20(1):324. doi: 10.1186/s12903-020-01313-1.
Proper oxygen balance in the dental pulp is essential for cell metabolism. Angiogenesis in the pulp is a constant process during the life of the tooth. Hypoxia indicators in a tissue, such as HIF-1α, as well as vascular destabilization markers, such as ANG2 and its receptor TIE2, are necessary for angiogenesis. Therefore the purpose of this study is to evaluate the expression of HIF-1α, ANG1, ANG2 and TIE2 in dental pulp as early angiogenesis indicators in teeth with complete and incomplete root development.
Forty human dental pulps were obtained from freshly extracted third molars divided into two groups: incomplete (n = 20) and complete (n = 20) root development. Dental pulps were stored at - 80 °C, defrosted in an ice bath and re-frozen with their respective thaws to disintegrate the tissue. Three sonication cycles were performed until the tissues were homogenized, then thaw were centrifuged and the supernatant was collected for the detection of the markers to be studied. The samples were processed for the ELISA test using the ELISA-sandwich principle. Student t and Mann-Whitney U tests were performed to determine statistically significant differences between groups.
In the complete root development, HIF-1α, ANG1, ANG2 and TIE2 expressions were significantly higher than their expression in the incomplete root development group.
The angiogenic process seems to be a physiological process in the dental pulp. Angiogenic activity is higher in teeth with mature than immature apex teeth.
牙髓内适当的氧气平衡对细胞代谢至关重要。牙髓内的血管生成是牙齿一生中持续不断的过程。组织中的缺氧指标，如 HIF-1α，以及血管不稳定标记物，如 ANG2 和其受体 TIE2，是血管生成所必需的。因此，本研究的目的是评估 HIF-1α、ANG1、ANG2 和 TIE2 在牙髓中的表达，作为有完整和不完全牙根发育的牙齿早期血管生成的指标。
从新鲜拔出的第三磨牙中获得 40 个人类牙髓，分为两组：不完全（n = 20）和完全（n = 20）牙根发育。牙髓在-80°C 下储存，在冰浴中解冻，并用各自的解冻液再冷冻以使组织解体。进行三个超声循环，直到组织均质化，然后解冻并离心，收集上清液用于检测待研究的标记物。使用 ELISA 夹心原理对样品进行 ELISA 测试。采用 Student t 和 Mann-Whitney U 检验对组间差异进行统计学分析。
在完全牙根发育中，HIF-1α、ANG1、ANG2 和 TIE2 的表达明显高于不完全牙根发育组。
血管生成过程似乎是牙髓中的一种生理过程。成熟根尖牙的血管生成活性高于不成熟根尖牙。
