Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, College of Life Science, Yangtze University, Jingzhou, 434025, China.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.
J Plant Physiol. 2019 Sep;240:153007. doi: 10.1016/j.jplph.2019.153007. Epub 2019 Jul 9.
Previous studies have proven that graphene oxide (GO) regulates abscisic acid (ABA) and indole-3-acetic acid (IAA) contents and modulates plant root growth. To better understand the mechanism of plant growth and development regulated by GO and crosstalk between ABA and GO, Zhongshuang No. 9 seedlings were treated with GO and ABA. The results indicated that GO and ABA significantly affected the morphological properties and endogenous phytohormone contents in seedlings, and there was significant crosstalk between GO and ABA. ABA treatments combined with GO led to a rapid decrease in triphenyltetrazolium chloride (TTC) reduction intensity, and the inhibitory effect was enhanced with increasing ABA concentration. The treatments significantly affected the transcriptional levels of some key genes involved in the ABA, IAA, cytokinin (CTK), salicylic acid (SA), and ethane (ETH) pathways and increased the ABA and gibberellin (GA) contents in rapeseed seedlings. The effects of the treatments on the IAA and CTK contents were complex, but, importantly, the treatments suppressed root elongation. Correlation analysis also indicated that the relationship between root length and IAA/ABA could be described by a polynomial function: y = 88.11x - 25.15x + 4.813(R² = 0.912). The treatments increased the ACS2 transcript abundance for ETH biosynthesis and the ICS1 transcriptional level of the key genes involved in salicylic acid (SA) biosynthesis, as well as the downstream signaling genes CBP60 and SARD1. This finding indicated that ABA is an important factor regulating the effects of GO on the growth and development of Brassica napus L., and that ETH and SA pathways may be potential pathways involved in the response of rape seedlings to GO treatment.
先前的研究已经证明，氧化石墨烯（GO）调节脱落酸（ABA）和吲哚-3-乙酸（IAA）的含量，并调节植物根的生长。为了更好地理解 GO 调节植物生长和发育的机制以及 ABA 与 GO 之间的串扰，我们用 GO 和 ABA 处理了中双 9 号幼苗。结果表明，GO 和 ABA 显著影响了幼苗的形态特征和内源植物激素含量，GO 和 ABA 之间存在显著的串扰。GO 和 ABA 联合处理导致三苯基四唑氯化物（TTC）还原强度迅速下降，随着 ABA 浓度的增加，抑制作用增强。这些处理显著影响了参与 ABA、IAA、细胞分裂素（CTK）、水杨酸（SA）和乙烷（ETH）途径的一些关键基因的转录水平，并增加了油菜幼苗中的 ABA 和赤霉素（GA）含量。处理对 IAA 和 CTK 含量的影响较为复杂，但重要的是，处理抑制了根的伸长。相关分析还表明，根长与 IAA/ABA 之间的关系可以用多项式函数来描述：y = 88.11x - 25.15x + 4.813(R² = 0.912)。这些处理增加了 ETH 生物合成的 ACS2 转录丰度和水杨酸（SA）生物合成关键基因 ICS1 的转录水平，以及下游信号基因 CBP60 和 SARD1。这一发现表明，ABA 是调节 GO 对油菜生长和发育影响的重要因素，ETH 和 SA 途径可能是油菜幼苗对 GO 处理反应的潜在途径。
