Tuhaise Samuel, Nakavuma Jesca L, Adriko John, Ssekatawa Kenneth, Kiggundu Andrew
National Agricultural Biotechnology Centre, National Agricultural Research Laboratories, Kawanda-NARO, P.O Box 7065, Kampala, Uganda.
College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.
BMC Res Notes. 2019 Jul 16;12(1):425. doi: 10.1186/s13104-019-4469-8.
Passion fruit improvement efforts by conventional breeding have had limited success calling for research into alternative approaches such as tissue culture and genetic engineering. An efficient and reproducible regeneration system is a prerequisite for successful genetic engineering. Currently, there is no reliable regeneration system for Uganda's passion fruit varieties owing to the high heterogeneity of the Passiflora genus. Therefore, this study aimed at establishing an efficient and reproducible regeneration system for Uganda's Passiflora edulis f. flavicarpa (yellow passion fruit) and Passiflora edulis f. edulis (purple passion fruit) for routine utilization with an ultimate goal of improving its agronomic value.
The study successfully induced shoots by both direct and indirect organogenesis for the yellow passion fruit variety. Highest shoot induction frequency (14.85%) was achieved on 8.9 μM BAP while 7.9 μM BAP did not initiate any shoots. Optimal shoot elongation and rooting was achieved on 0.44 μM BAP and 5.37 µM α-naphthaleneacetic (NAA) respectively. Rooted yellow passion fruit plantlets were successfully weaned with over 65% survival rates. It took approximately 6 months to produce a weaned healthy passion fruit plant. The purple passion fruit variety proved to be recalcitrant to tissue culture with no successful shoot or callus induction.
通过传统育种改良西番莲成效有限，因此需要研究组织培养和基因工程等替代方法。高效且可重复的再生系统是基因工程成功的前提条件。目前，由于西番莲属高度异质性，乌干达的西番莲品种尚无可靠的再生系统。因此，本研究旨在为乌干达的黄果西番莲（黄百香果）和紫果西番莲（紫百香果）建立高效且可重复的再生系统，以便常规利用，最终目标是提高其农艺价值。
该研究通过直接和间接器官发生成功诱导出黄百香果品种的芽。在8.9 μM 苄氨基嘌呤（BAP）上获得了最高的芽诱导频率（14.85%），而7.9 μM BAP未诱导出任何芽。分别在0.44 μM BAP和5.37 μM α-萘乙酸（NAA）上实现了最佳的芽伸长和生根。生根的黄百香果植株成功驯化，成活率超过65%。培育出一株驯化后的健康百香果植株大约需要6个月。紫百香果品种对组织培养具有抗性，未成功诱导出芽或愈伤组织。
