Chen Jiayi, He Fangting, Liu Sijing, Zhou Tao, Baloch Saira, Jiang Chunping, Pei Xiaofang
West China School of Public Health and West China Fourth Hospital, Sichuan University, 16#, Section 3, Renmin Road South, Chengdu 610041, Sichuan, China.
Evid Based Complement Alternat Med. 2019 Jun 13;2019:5026458. doi: 10.1155/2019/5026458. eCollection 2019.
is a traditional herbal tea that is widely distributed in southwest China. The health effects of are characteristics of clearing heat, antioxidant, inducing resurgence, and improving digestion. However, the molecular mechanisms related to these effects, particularly the antioxidant mechanism, have been seldom reported. The objective of this study was to assess antioxidative capacity of , and its protective effects and mechanisms against hydrogen peroxide (HO) - induced toxicity in Caco-2 cells. Total phenolic contents, free radical scavenging activity, and reducing capacity of were measured. The effects of on the cell viability and antioxidant defense system were explored. The expression of nuclear factor E2 related factor 2 (Nrf2) and antioxidant genes: quinone oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1), and glutamate cysteine ligase (GCL) were analyzed by western blot and qPCR. Pretreatment of could significantly reduce HO-induced toxicity, decrease the level of reactive oxygen species (ROS) and malondialdehyde (MDA), and increase the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione reductase (GR). By activating the expression of Nrf2 and antioxidant genes (NQO1, HO-1, and GCL), exerts cytoprotective effect in Caco-2 cells dealt with HO. Therefore, the well-established model of Caco-2 cells demonstrates that may modulate the cytoprotective effect against the HO-induced oxidative stress through the Nrf2 signaling pathway.
是一种广泛分布于中国西南部的传统凉茶。其健康功效具有清热、抗氧化、促复苏和改善消化的特点。然而，与这些功效相关的分子机制，尤其是抗氧化机制，鲜有报道。本研究的目的是评估其抗氧化能力，以及其对过氧化氢（HO）诱导的Caco-2细胞毒性的保护作用和机制。测定了其总酚含量、自由基清除活性和还原能力。探讨了其对细胞活力和抗氧化防御系统的影响。通过蛋白质免疫印迹法和定量聚合酶链反应分析了核因子E2相关因子2（Nrf2）和抗氧化基因：醌氧化还原酶1（NQO1）、血红素加氧酶-1（HO-1）和谷氨酸半胱氨酸连接酶（GCL）的表达。预处理可显著降低HO诱导的毒性，降低活性氧（ROS）和丙二醛（MDA）水平，并增加超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽还原酶（GR）的活性。通过激活Nrf2和抗氧化基因（NQO1、HO-1和GCL）的表达，在处理HO的Caco-2细胞中发挥细胞保护作用。因此，成熟的Caco-2细胞模型表明，可能通过Nrf2信号通路调节对HO诱导的氧化应激的细胞保护作用。
