Bhandari Adheesh, Guan Yaoyao, Xia Erjie, Huang Qidi, Chen Yizuo
Department of Thyroid & Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, Zhejiang, PR China.
Am J Transl Res. 2019 Jun 15;11(6):3589-3599. eCollection 2019.
Thyroid cancer incidence has been continuity growing globally. To Find reliable molecular biomarkers to assess prognosis and select optimal treatment is necessary. VASN is a protein-coding gene that plays a vital part in tumor development and angiogenesis. Analyzing the TCGA dates, we found VASN could be a potential marker in assessing thyroid prognosis. The act of VASN in thyroid cancer is not explicit. In this article, we investigate the function of VASN expression in thyroid cancer.
The Cancer Genome Atlas (TCGA) unpaired thyroid cancer and normal RNA-seq data was download and our paired thyroid cancer, and a polymerase chain reaction analyzed normal samples. The expression of VASN was regulated by transfected small interfering RNA, and the function of VASN was determined through migration, invasion and cell proliferation assays. Western blot assay was performed to reveal the relation between the VASN expression and YAP/TAZ pathway, epithelial-mesenchymal transition in thyroid carcinogenesis.
The significant upregulation of VASN in papillary thyroid carcinoma tissues associated to normal thyroid tissues was revealed by our data and TCGA data. VASN overexpression was significantly correlated to lymph node metastasis, tumor stage and tumor size. In the cell, experiments showed that VASN low expression significantly suppressed the migration, invasion, and proliferation. Western blot assay proves the effect of VASN expression on YAP/TAZ pathway and epithelial-mesenchymal transition.
VASN plays a crucial oncogene in thyroid cancer. Our results indicate that VASN could be a biomarker of thyroid cancer and may act in the YAP/TAZ pathway to regulate epithelial-mesenchymal transition (EMT).
全球范围内甲状腺癌的发病率一直在持续上升。寻找可靠的分子生物标志物以评估预后并选择最佳治疗方案很有必要。VASN是一个蛋白质编码基因，在肿瘤发展和血管生成中起着至关重要的作用。通过分析TCGA数据，我们发现VASN可能是评估甲状腺癌预后的一个潜在标志物。VASN在甲状腺癌中的作用尚不明确。在本文中，我们研究了VASN表达在甲状腺癌中的功能。
下载癌症基因组图谱（TCGA）中未配对的甲状腺癌和正常RNA测序数据，并对我们配对的甲状腺癌及正常样本进行聚合酶链反应分析。通过转染小干扰RNA来调节VASN的表达，并通过迁移、侵袭和细胞增殖实验来确定VASN的功能。进行蛋白质印迹分析以揭示VASN表达与YAP/TAZ通路、甲状腺癌发生过程中的上皮-间质转化之间的关系。
我们的数据和TCGA数据均显示，与正常甲状腺组织相比，甲状腺乳头状癌组织中VASN显著上调。VASN过表达与淋巴结转移、肿瘤分期和肿瘤大小显著相关。在细胞实验中，结果表明VASN低表达显著抑制了迁移、侵袭和增殖。蛋白质印迹分析证实了VASN表达对YAP/TAZ通路和上皮-间质转化的影响。
VASN在甲状腺癌中起着关键的癌基因作用。我们的结果表明，VASN可能是甲状腺癌的一个生物标志物，并且可能在YAP/TAZ通路中发挥作用以调节上皮-间质转化（EMT）。
