Department of Thyroid and Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
J Cell Mol Med. 2019 Nov;23(11):7438-7448. doi: 10.1111/jcmm.14612. Epub 2019 Sep 9.
Thyroid cancer incidence has been continuity increasing worldwide. Uridine phosphorylase 1 (UPP1) is a protein-coding gene and has been detected that UPP1 was the higher expression in many solid malignancies, just as head and neck cancers, breast cancer, compared with paired normal tissue. But the act of UPP1 in thyroid cancer is not explicit. In this article, we investigate the function of UPP1 expression in thyroid cancer. The Cancer Genome Atlas (TCGA) unpaired thyroid cancer and normal RNA-seq data were downloaded, and our paired thyroid cancer and normal samples were analysed by a polymerase chain reaction. The expression of UPP1 was regulated by transfected small interfering RNA, and the function of UPP1 was determined via migration, invasion and cell proliferation assays. Western blot assay was achieved to determine the UPP1 expression correlates with the function of 5-FU regulate epithelial-mesenchymal transition. The significant upregulation of UPP1 in thyroid cancer tissues compared with normal thyroid tissues was revealed by our data and TCGA data. UPP1 overexpression was significantly correlated with lymph node metastasis, tumour stage and tumour size. In the cell, experiments showed that UPP1 low expression significantly suppressed the migration, invasion and proliferation. Western blot assay proves the effect of UPP1 expression on 5-FU regulates epithelial-mesenchymal transition pathway. UPP1 plays a crucial oncogene in thyroid cancer. Our findings indicate that UPP1 might be a biomarker of thyroid cancer and may act by regulating epithelial-mesenchymal transition (EMT).
甲状腺癌的发病率在全球范围内持续增长。尿苷磷酸化酶 1（UPP1）是一种蛋白质编码基因，已发现 UPP1 在许多实体恶性肿瘤中表达较高，如头颈部癌症、乳腺癌，与配对的正常组织相比。但 UPP1 在甲状腺癌中的作用尚不清楚。本文研究了 UPP1 表达在甲状腺癌中的作用。下载了癌症基因组图谱（TCGA）未配对的甲状腺癌和正常 RNA-seq 数据，并通过聚合酶链反应分析了我们配对的甲状腺癌和正常样本。通过转染小干扰 RNA 调节 UPP1 的表达，通过迁移、侵袭和细胞增殖测定来确定 UPP1 的功能。通过 Western blot 测定确定 UPP1 的表达与 5-FU 调节上皮-间充质转化的功能相关。我们的数据和 TCGA 数据显示，甲状腺癌组织中 UPP1 的表达明显高于正常甲状腺组织。UPP1 的过表达与淋巴结转移、肿瘤分期和肿瘤大小显著相关。在细胞实验中，结果表明 UPP1 低表达显著抑制了迁移、侵袭和增殖。Western blot 测定证明了 UPP1 表达对 5-FU 调节上皮-间充质转化途径的影响。UPP1 在甲状腺癌中起着关键的癌基因作用。我们的研究结果表明，UPP1 可能是甲状腺癌的一个生物标志物，并可能通过调节上皮-间充质转化（EMT）发挥作用。
