人肝内胆管上皮细胞的分离与原代培养
Isolation and primary cultures of human intrahepatic bile ductular epithelium.
作者信息
Demetris A J, Markus B H, Saidman S, Fung J J, Makowka L, Graner S, Duquesnoy R, Starzl T E
机构信息
Department of Pathology, Presbyterian University Hospital, Pittsburgh, PA.
出版信息
In Vitro Cell Dev Biol. 1988 May;24(5):464-70. doi: 10.1007/BF02628499.
Abstract
A technique for the isolation of human intrahepatic bile ductular epithelium, and the establishment of primary cultures using a serum- and growth-factor-supplemented medium combined with a connective tissue substrata is described. Initial cell isolates and monolayer cultures display phenotypic characteristics of biliary epithelial cells (low molecular weight prekeratin positive; albumin, alphafetoprotein, and Factor VIII-related antigen negative). Ultrastructural features of the cultured cells show cell polarization with surface microvilli, numerous interepithelial junctional complexes and cytoplasmic intermediate prekeratin filaments.
摘要
本文描述了一种分离人肝内胆管上皮细胞的技术，以及使用添加血清和生长因子的培养基并结合结缔组织基质建立原代培养的方法。最初分离的细胞和单层培养物表现出胆管上皮细胞的表型特征（低分子量前角蛋白阳性；白蛋白、甲胎蛋白和VIII因子相关抗原阴性）。培养细胞的超微结构特征显示细胞极化，有表面微绒毛、大量上皮间连接复合体和细胞质中间前角蛋白丝。
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本文引用的文献
1
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Exp Mol Pathol. 1964 Jun;3:242-61. doi: 10.1016/0014-4800(64)90057-7.
2
Morphologic identification by electron microscopy of "oval" cells in experimental hepatic degeneration.实验性肝变性中“卵圆”细胞的电子显微镜形态学鉴定
Lab Invest. 1961 Mar-Apr;10:317-32.
3
Cell types in long-term propagable cultures of rat liver.大鼠肝脏长期可传代培养中的细胞类型。
Ann N Y Acad Sci. 1980;349:128-37. doi: 10.1111/j.1749-6632.1980.tb29521.x.
4
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Am J Pathol. 1983 Mar;110(3):322-32.
5
6
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Proc Natl Acad Sci U S A. 1984 Sep;81(17):5435-9. doi: 10.1073/pnas.81.17.5435.
7
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Exp Cell Res. 1984 Sep;154(1):38-52. doi: 10.1016/0014-4827(84)90666-9.
8
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