School of Food and Biological Engineering, Jiangsu Univ., Zhenjiang, 212013, China.
Inst. of Food Physical Processing, Jiangsu Univ., Zhenjiang, 212013, China.
J Food Sci. 2019 Aug;84(8):2139-2146. doi: 10.1111/1750-3841.14720. Epub 2019 Jul 17.
Wheat germ protein (WGP) was extracted with subcritical water and then hydrolyzed with Alcalase 2.4 L to obtain antioxidant hydrolysates. Wheat germ peptides (WG-P, Mw < 1 kDa) were purified by using Sephadex G-15 column chromatography. The results showed that WG-P-4 possessed the strongest DPPH radical scavenging activity in comparison with other peptides fractions. In addition, free amino acids and LC-MS/MS analysis showed that Gly-Pro-Phe, Gly-Pro-Glu, and Phe-Gly-Glu were the major peptides of WG-P-4. Interestingly, the WG-P-4 fractions had good absorption characteristic. Moreover, the ratio of P both sides of apical compartment (AP) and basolateral compartment (BL) were between 0.5 and 1.0 on Caco-2 cell model, which indicated that transmembrane transportation was mainly passive transport. Therefore, WG-P could exert an effective antioxidant action by across the intestinal epithelium.
小麦胚芽蛋白（WGP）用亚临界水提取，然后用 Alcalase 2.4 L 水解得到抗氧化水解产物。用 Sephadex G-15 柱层析法对小麦胚芽肽（WG-P，Mw < 1 kDa）进行纯化。结果表明，与其他肽段相比，WG-P-4 具有最强的 DPPH 自由基清除活性。此外，游离氨基酸和 LC-MS/MS 分析表明，Gly-Pro-Phe、Gly-Pro-Glu 和 Phe-Gly-Glu 是 WG-P-4 的主要肽段。有趣的是，WG-P-4 馏分具有良好的吸收特性。此外，在 Caco-2 细胞模型中，AP 和 BL 两侧的 P 比值在 0.5 到 1.0 之间，这表明跨膜转运主要是被动转运。因此，WG-P 可以通过穿过肠上皮发挥有效的抗氧化作用。
