College of Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
Beijing Engineering Research Center of Protein and Functional Peptides, China National Research Institute of Food and Fermentation Industries Co., Ltd, Beijing, 100015, People's Republic of China.
Sci Rep. 2021 Mar 4;11(1):5206. doi: 10.1038/s41598-021-84820-7.
This study aimed to focus on the high-value utilization of raw wheat gluten by determining the potent antioxidant peptides and angiotensin I-converting enzyme (ACE) inhibitory peptides from wheat gluten oligopeptides (WOP). WOP were analyzed for in vitro antioxidant activity and inhibition of ACE, and the identification of active peptides was performed by reversed-phase high-performance liquid chromatography and mass spectrometry. Quantitative analysis was performed for highly active peptides. Five potent antioxidant peptides, Leu-Tyr, Pro-Tyr, Tyr-Gln, Ala-Pro-Ser-Tyr and Arg-Gly-Gly-Tyr (6.07 ± 0.38, 7.28 ± 0.29, 11.18 ± 1.02, 5.93 ± 0.20 and 9.04 ± 0.47 mmol 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) equivalent/g sample, respectively), and five potent ACE inhibitory peptides, Leu-Tyr, Leu-Val-Ser, Tyr-Gln, Ala-Pro-Ser-Tyr and Arg-Gly-Gly-Tyr (half maximal inhibitory concentration (IC) values = 0.31 ± 0.02, 0.60 ± 0.03, 2.00 ± 0.13, 1.47 ± 0.08 and 1.48 ± 0.11 mmol/L, respectively), were observed. The contents of Leu-Tyr, Pro-Tyr, Tyr-Gln, Ala-Pro-Ser-Tyr, Arg-Gly-Gly-Tyr, and Leu-Val-Ser were 155.04 ± 8.36, 2.08 ± 0.12, 1.95 ± 0.06, 22.70 ± 1.35, 0.25 ± 0.01, and 53.01 ± 2.73 μg/g, respectively, in the WOP. Pro-Tyr, Tyr-Gln, Ala-Pro-Ser-Tyr, Arg-Gly-Gly-Tyr, and Leu-Val-Ser are novel antioxidative/ACE inhibitory peptides that have not been previously reported. The results suggest that WOP could potentially be applied in the food industry as a functional additive.
本研究旨在通过确定小麦面筋寡肽（WOP）中的有效抗氧化肽和血管紧张素转化酶（ACE）抑制肽，来关注小麦面筋的高价值利用。对 WOP 进行体外抗氧化活性和 ACE 抑制分析，并通过反相高效液相色谱和质谱法对活性肽进行鉴定。对高活性肽进行定量分析。鉴定出五种具有较强抗氧化活性的肽，分别为亮氨酸-酪氨酸（Leu-Tyr）、脯氨酸-酪氨酸（Pro-Tyr）、酪氨酸-谷氨酰胺（Tyr-Gln）、丙氨酸-脯氨酸-丝氨酸-酪氨酸（Ala-Pro-Ser-Tyr）和精氨酸-甘氨酸-甘氨酸-酪氨酸（Arg-Gly-Gly-Tyr）（6.07±0.38、7.28±0.29、11.18±1.02、5.93±0.20 和 9.04±0.47mmol 6-羟基-2,5,7,8-四甲基色满-2-羧酸（Trolox）当量/g 样品），以及五种具有较强 ACE 抑制活性的肽，分别为亮氨酸-酪氨酸（Leu-Tyr）、亮氨酸-缬氨酸-丝氨酸（Leu-Val-Ser）、酪氨酸-谷氨酰胺（Tyr-Gln）、丙氨酸-脯氨酸-丝氨酸-酪氨酸（Ala-Pro-Ser-Tyr）和精氨酸-甘氨酸-甘氨酸-酪氨酸（Arg-Gly-Gly-Tyr）（半最大抑制浓度（IC）值分别为 0.31±0.02、0.60±0.03、2.00±0.13、1.47±0.08 和 1.48±0.11mmol/L）。在 WOP 中，亮氨酸-酪氨酸（Leu-Tyr）、脯氨酸-酪氨酸（Pro-Tyr）、酪氨酸-谷氨酰胺（Tyr-Gln）、丙氨酸-脯氨酸-丝氨酸-酪氨酸（Ala-Pro-Ser-Tyr）、精氨酸-甘氨酸-甘氨酸-酪氨酸（Arg-Gly-Gly-Tyr）和亮氨酸-缬氨酸-丝氨酸（Leu-Val-Ser）的含量分别为 155.04±8.36、2.08±0.12、1.95±0.06、22.70±1.35、0.25±0.01 和 53.01±2.73μg/g。脯氨酸-酪氨酸（Pro-Tyr）、酪氨酸-谷氨酰胺（Tyr-Gln）、丙氨酸-脯氨酸-丝氨酸-酪氨酸（Ala-Pro-Ser-Tyr）、精氨酸-甘氨酸-甘氨酸-酪氨酸（Arg-Gly-Gly-Tyr）和亮氨酸-缬氨酸-丝氨酸（Leu-Val-Ser）是以前没有报道过的新型抗氧化/ACE 抑制肽。结果表明，WOP 有可能作为一种功能性添加剂应用于食品工业。
