Ahmad Rahaf M, Greish Yaser E, El-Maghraby Hesham F, Lubbad Loay, Makableh Yahia, Hammad Fayez T
Department of Surgery, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab Emirates.
Institute of Nanotechnology, Jordan University of Science and Technology, Irbid 22110, Jordan.
Nanomaterials (Basel). 2022 Apr 1;12(7):1183. doi: 10.3390/nano12071183.
Recently, there has been a growing interest in using natural products as treatment alternatives in several diseases. Nerolidol is a natural product which has been shown to have protective effects in several conditions. The low water solubility of nerolidol and many other natural products limits their delivery to the body. In this research, a drug delivery system composed of alginate and chitosan was fabricated and loaded with nerolidol to enhance its water solubility. The chitosan-alginate nanoparticles were fabricated using a new method including the tween 80 pre-gelation, followed by poly-ionic crosslinking between chitosan negative and alginate positive groups. Several characterization techniques were used to validate the fabricated nanoparticles. The molecular interactions between the chitosan, alginate, and nerolidol molecules were confirmed using the Fourier transform infrared spectroscopy. The ultraviolet spectroscopy showed an absorbance peak of the blank nanoparticles at 200 nm and for the pure nerolidol at 280 nm. Using both scanning and transmission electron microscopy, the nanoparticles were found to be spherical in shape with an average size of 12 nm and 35 nm for the blank chitosan-alginate nanoparticles and the nerolidol-loaded chitosan-alginate nanoparticles, respectively. The nanoparticles were also shown to have a loading capacity of 51.7% and an encapsulation efficiency of 87%. A controlled release profile of the loaded drug for up to 28 h using an in vitro model was also observed, which is more efficient than the free form of nerolidol. In conclusion, chitosan-alginate nanoparticles and nerolidol loaded chitosan-alginate nanoparticles were successfully fabricated and characterized to show potential encapsulation and delivery using an in vitro model.
近年来，人们越来越关注将天然产物用作多种疾病的治疗替代方案。橙花叔醇是一种天然产物，已被证明在多种情况下具有保护作用。橙花叔醇和许多其他天然产物的低水溶性限制了它们进入人体。在本研究中，制备了一种由海藻酸盐和壳聚糖组成的药物递送系统，并负载橙花叔醇以提高其水溶性。壳聚糖-海藻酸盐纳米颗粒采用一种新方法制备，包括吐温80预凝胶化，然后在壳聚糖负离子和海藻酸盐正离子基团之间进行聚离子交联。使用了几种表征技术来验证制备的纳米颗粒。利用傅里叶变换红外光谱证实了壳聚糖、海藻酸盐和橙花叔醇分子之间的分子相互作用。紫外光谱显示空白纳米颗粒在200 nm处有吸收峰，纯橙花叔醇在280 nm处有吸收峰。通过扫描电子显微镜和透射电子显微镜发现，空白壳聚糖-海藻酸盐纳米颗粒和负载橙花叔醇的壳聚糖-海藻酸盐纳米颗粒的形状均为球形，平均尺寸分别为12 nm和35 nm。纳米颗粒的载药量为51.7%，包封率为87%。还观察到使用体外模型时负载药物的控释曲线长达28小时，这比游离形式的橙花叔醇更有效。总之，成功制备并表征了壳聚糖-海藻酸盐纳米颗粒和负载橙花叔醇的壳聚糖-海藻酸盐纳米颗粒，以显示其在体外模型中的潜在包封和递送能力。
