Department of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Periodontology, University of Medicine and Pharmacy, Craiova, Romania.
Microsc Res Tech. 2019 Oct;82(10):1768-1778. doi: 10.1002/jemt.23343. Epub 2019 Jul 16.
Resin composite-based dental materials can leach certain components into the oral environment, causing potentially harmful gingival biological effect. Gingival tissue is a rich source of mesenchymal stem cells (MSCs) that is easily accessible, and can be used as a complementary approach for the investigation of dental material biocompatibility. Using gingival MSCs (gMSCs), the present study aimed to investigate the cytotoxicity of two classes of restorative dental materials (ormocers and resin composites) used to restore class II cavities close to the gingival margin, in addition to analyzing the leached compounds from these resin composite-based materials. Functionality assays (Colony-forming unit, migratory potential, and proliferation assays) and a viability assay (MTT) were employed. Cells' aspect was observed by scanning electron microscopy (SEM). Leached monomers were also quantitated using high-performance liquid chromatography (HPLC). The cytotoxicity of the biomaterials was highlighted by impaired functionality and diminished viability of gMSCs. Despite being variants of the same commercial material, the two ormocers behaved differently one material having a more negative impact on cell functionality than the other. Cells appeared to attach well to all materials. Main monomer molecules were mostly released by the tested materials. For all samples, an increased elution of monomers was recorded in artificial saliva as compared with culture medium. One composite material has released nearly eight times more urethane dimetacrylate in artificial saliva than in culture medium. Significantly lower gMSC viability scores were recorded for all the investigated samples in comparison with the control.
基于树脂的牙科复合材料会将某些成分浸出到口腔环境中，从而对牙龈组织产生潜在的有害生物学效应。牙龈组织是间充质干细胞（MSCs）的丰富来源，这些细胞容易获取，可用于研究牙科材料的生物相容性。本研究使用牙龈间充质干细胞（gMSCs），旨在研究用于修复接近牙龈缘的 II 类牙洞的两类修复性牙科材料（有机硅烷和树脂复合材料）的细胞毒性，同时分析这些基于树脂复合材料的浸出化合物。采用功能测定法（集落形成单位、迁移潜力和增殖测定法）和活力测定法（MTT）进行研究。通过扫描电子显微镜（SEM）观察细胞形态。采用高效液相色谱法（HPLC）定量测定浸出的单体。生物材料的细胞毒性表现为 gMSCs 的功能受损和活力下降。尽管这两种有机硅烷属于同一商业材料的变体，但其中一种材料对细胞功能的负面影响大于另一种。细胞似乎很好地附着在所有材料上。主要单体分子主要由测试材料释放。与培养基相比，所有样品在人工唾液中的单体洗脱量均增加。一种复合材料在人工唾液中的释放量比在培养基中多释放了近 8 倍的异氰酸酯二甲基丙烯酸酯。与对照组相比，所有被调查样本的 gMSC 活力评分明显较低。
