Walther-Straub-Institute of Pharmacology and Toxicology, Ludwig-Maximilians-University of Munich, Nussbaumstrasse 26, D-80336 Munich, Germany.
Biomaterials. 2010 Mar;31(8):2010-4. doi: 10.1016/j.biomaterials.2009.11.065. Epub 2009 Dec 9.
Dental resin composites and their reactive monomers/co-monomers have been shown to elicit cytotoxic responses in human gingival fibroblasts (HGF), and their metabolic radical intermediates have the potential to attack the DNA backbone, which may induce DNA double-strand breaks (DSBs). In this study we have tested the cytotoxicity and induction of DSBs by the most common composite resin monomers/co-monomers: BisGMA, HEMA, TEGDMA, and UDMA in gingival fibroblasts using the sensitive gamma-H2AX DNA repair focus assay. Our results show increasing monomer cytotoxicities in the order of BisGMA>UDMA>TEGDMA>HEMA, an order that was also observed for their capacity to induce DSBs. BisGMA at the EC50 concentration of 0.09 mm evoked the highest rate of gamma-H2AX foci-formation that was 11-fold higher DNA DSBs as compared to the negative controls that ranged between 0.25 and 0.5gamma-H2AX foci/HGF cell. Our results for the first time show that exposure to dental resin monomers can induce DSBs in primary human oral cavity cells, which underscores their genotoxic capacity.
牙科树脂复合材料及其反应性单体/共聚单体已被证明会在人牙龈成纤维细胞（HGF）中引起细胞毒性反应，其代谢自由基中间体有可能攻击 DNA 骨架，从而可能诱导 DNA 双链断裂（DSB）。在这项研究中，我们使用灵敏的γ-H2AX DNA 修复焦点测定法，测试了最常见的复合树脂单体/共聚单体：BisGMA、HEMA、TEGDMA 和 UDMA 在牙龈成纤维细胞中的细胞毒性和 DSB 诱导作用。我们的结果表明，单体的细胞毒性按 BisGMA>UDMA>TEGDMA>HEMA 的顺序增加，这与它们诱导 DSB 的能力顺序一致。BisGMA 在 EC50 浓度为 0.09mm 时引发的γ-H2AX 焦点形成率最高，比阴性对照高出 11 倍，阴性对照的范围在 0.25 到 0.5 个γ-H2AX 焦点/HGF 细胞之间。我们的研究结果首次表明，暴露于牙科树脂单体可在人口腔原发性细胞中诱导 DSB，这突出了它们的遗传毒性。
