针对整合素β1亚基保守胞质结构域的抗体可与脊椎动物、无脊椎动物及真菌中的蛋白质发生反应。
Antibodies to the conserved cytoplasmic domain of the integrin beta 1 subunit react with proteins in vertebrates, invertebrates, and fungi.
作者信息
Marcantonio E E, Hynes R O
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
J Cell Biol. 1988 May;106(5):1765-72. doi: 10.1083/jcb.106.5.1765.
Abstract
The integrin family of cell surface receptors can be divided into three groups on the basis of their homologous beta subunits: beta 1, beta 2, and beta 3. We have raised an antibody against a synthetic peptide corresponding to the COOH-terminal domain of the chicken integrin beta 1 subunit that reacts with beta subunits from a variety of vertebrates, invertebrates, and fungi, demonstrating strong evolutionary conservation of sequences in this domain. In Drosophila cells, the antibody recognizes integrin alpha beta complexes that appear to be identical with position-specific antigens. Cross-reactive proteins are also detected in Caenorhabditis elegans and Candida albicans. The antiserum is specific for beta 1 subunits and does not recognize other integrin beta subunits in humans. In immunofluorescence analyses of cultured cells, the antibody reacts only with permeabilized cells confirming that this highly conserved COOH-terminal segment is a cytoplasmic domain.
摘要
细胞表面受体的整合素家族可根据其同源β亚基分为三组：β1、β2和β3。我们制备了一种针对与鸡整合素β1亚基COOH末端结构域相对应的合成肽的抗体，该抗体可与多种脊椎动物、无脊椎动物和真菌的β亚基发生反应，表明该结构域中的序列具有很强的进化保守性。在果蝇细胞中，该抗体识别的整合素αβ复合物似乎与位置特异性抗原相同。在秀丽隐杆线虫和白色念珠菌中也检测到了交叉反应蛋白。该抗血清对β1亚基具有特异性，不识别人类中的其他整合素β亚基。在培养细胞的免疫荧光分析中，该抗体仅与通透化细胞发生反应，证实这个高度保守的COOH末端片段是一个胞质结构域。
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