1 Universite´ de Lille, Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), École Nationale Supérieure de Chimie de Lille (ENSCL), Unités Mixtes de Recherche (UMR) 8207-Unité Matériaux et Transformations (UMET)-Processus aux Interfaces et Hygiène des Matériaux (PIHM), 59000 Lille, France.
2 Universite´ de Lille, CNRS, INRA, ENSCL, UMR 8207-UMET-ISP (Inge´nierie des Syste`me Polyme`res), 59000 Lille, France.
J Food Prot. 2019 Aug;82(8):1357-1363. doi: 10.4315/0362-028X.JFP-18-565.
The aim of the present work was to study and compare the effect of growth temperature (20, 30, and 37°C) and surface type (stainless steel and polycarbonate) on the production of virulence factors, such as proteases and siderophores, and the risk of surface contamination associated with biofilm and planktonic cells. The increase of growth temperature from 20 to 37°C increased (approximately twofold) the electronegative charge and the hydrophobicity of the biofilm cell surface. biofilm cell adhesion to stainless steel and polycarbonate was 5- and 1.5-fold higher than their planktonic counterparts at 20 and 30°C, respectively. The increase of growth temperature from 20 to 37°C increased the production of proteases (twofold) and siderophores (twofold) and the cytotoxicity (up to 30-fold) against the HeLa cell line in the supernatants of planktonic and biofilm cultures. This study also highlighted that biofilm and planktonic cells exhibited distinct physiological properties with respect to the production of virulence factors and the cytotoxicity against the Hela cell line. Therefore, effective disinfection procedures should be adapted to inactivate bacteria detached from biofilms.
本研究旨在研究和比较生长温度（20°C、30°C 和 37°C）和表面类型（不锈钢和聚碳酸酯）对产毒因子（如蛋白酶和铁载体）的影响，以及与生物膜和浮游细胞相关的表面污染风险。与 20°C 和 30°C 相比，生长温度从 20°C 升高到 37°C 使生物膜细胞表面的负电性和疏水性增加了（约两倍）。在 20°C 和 30°C 时，生物膜细胞对不锈钢和聚碳酸酯的黏附率分别比浮游细胞高 5 倍和 1.5 倍。生长温度从 20°C 升高到 37°C 使浮游和生物膜培养物上清液中的蛋白酶（增加两倍）和铁载体（增加两倍）的产量以及对 HeLa 细胞系的细胞毒性（增加 30 倍）增加。本研究还强调了生物膜和浮游细胞在产毒因子的产生和对 HeLa 细胞系的细胞毒性方面表现出不同的生理特性。因此，应采用有效的消毒程序来灭活从生物膜中分离出来的细菌。
