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孵育持续时间、生长温度和非生物表面类型对生物膜分离的金黄色葡萄球菌细胞的细胞表面特性、粘附和致病性的影响。

Effect of incubation duration, growth temperature, and abiotic surface type on cell surface properties, adhesion and pathogenicity of biofilm-detached Staphylococcus aureus cells.

作者信息

Khelissa Simon Oussama, Jama Charafeddine, Abdallah Marwan, Boukherroub Rabah, Faille Christine, Chihib Nour-Eddine

机构信息

CNRS, ENSCL, UMR 8207-UMET-PSI, Université de Lille1, Avenue Dimitri Mendeleïev, CS 90108, 59652, Villeneuve d'Ascq, France.

CNRS, INRA, UMR 8207-UMET-PIHM, Université de Lille1, 369 rue Jules Guesde, CS 20039, 59651, Villeneuve d'Ascq, France.

出版信息

AMB Express. 2017 Oct 24;7(1):191. doi: 10.1186/s13568-017-0492-0.

DOI:10.1186/s13568-017-0492-0
PMID:29067567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5655377/
Abstract

The goal of this study was to investigate the effect of growth conditions such as the temperature (20, 30 and 37 °C), incubation duration (24 and 48 h) and surface type (stainless steel and polycarbonate) on the cell surface physicochemical properties and adhesion to abiotic surfaces of biofilm-detached and planktonic Staphylococcus aureus cells. This study tested also the hypothesis that S. aureus planktonic cells exhibit distinct pathogenic properties compared with their sessile counterparts. The results showed that the changes of the growth conditions promoted changes in the zeta potential, hydrophobicity, electron donor/acceptor character of the studied cell populations. Biofilm-detached cells showed a greater adhesion to stainless steel and polycarbonate compared with planktonic cells. Compared with planktonic cells, sessile ones showed higher cytotoxic effect against HeLa cells, DNase activity, and siderophore levels. The higher cytotoxic effect and production of DNase and siderophore increased with the increase of temperature and duration of incubations. Based on the obtained data, the S. aureus biofilm-detached cells were found to be distinct in many physiological properties compared with their planktonic counterparts.

摘要

本研究的目的是调查诸如温度（20、30和37°C）、培养持续时间（24和48小时）以及表面类型（不锈钢和聚碳酸酯）等生长条件对生物膜分离的和浮游的金黄色葡萄球菌细胞的细胞表面物理化学性质以及对非生物表面粘附性的影响。本研究还检验了一个假设，即与固着态金黄色葡萄球菌细胞相比，浮游态金黄色葡萄球菌细胞表现出不同的致病特性。结果表明，生长条件的变化促使所研究细胞群体的zeta电位、疏水性、电子供体/受体特性发生改变。与浮游细胞相比，生物膜分离的细胞对不锈钢和聚碳酸酯表现出更强的粘附性。与浮游细胞相比，固着态细胞对HeLa细胞表现出更高的细胞毒性作用、DNase活性和铁载体水平。随着温度和培养持续时间的增加，细胞毒性作用以及DNase和铁载体的产生增加。基于所获得的数据，发现生物膜分离的金黄色葡萄球菌细胞在许多生理特性方面与其浮游态对应细胞不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/8b4720308c6a/13568_2017_492_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/aab0a019d5a7/13568_2017_492_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/140150b4ecd0/13568_2017_492_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/0d401d102e71/13568_2017_492_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/0756fa7bc803/13568_2017_492_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/69d7de259113/13568_2017_492_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/8b4720308c6a/13568_2017_492_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/aab0a019d5a7/13568_2017_492_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/140150b4ecd0/13568_2017_492_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/0d401d102e71/13568_2017_492_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/0756fa7bc803/13568_2017_492_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/69d7de259113/13568_2017_492_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7415/5655377/8b4720308c6a/13568_2017_492_Fig6_HTML.jpg

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