College of Life and Health Science, Anhui Science and Technology University, Fengyang, Anhui 233100, China.
FEMS Microbiol Lett. 2019 Jul 1;366(13). doi: 10.1093/femsle/fnz163.
Malachite green (MG), as a triarylmethane compound, poses a health hazard and causes considerable environmental concern. In this work, batch biosorption experiments were conducted under different operational conditions such as pH, contact time and adsorption dose to assess the optimal parameters of MG dye removal by yeast biomass from aqueous solutions. Then, the conventional biochemical assay was used to evaluate MG removal efficiency (75.18 and 95.85%) by Saccharomyces cerevisiae and Candida utilis. In addition, Fourier-transform infrared spectroscopy in combination with Raman microspectroscopy was employed to scrutinize the differences of dye removal between two types of yeast strains. This study demonstrates that Raman microspectroscopy may serve as a useful and powerful tool to quantitatively measure the content of MG dye on yeast cell surfaces in situ, and even offer an alternative new technique to seek potentially proper adsorbents for the removal of toxic dyes from industrial effluents.
孔雀石绿（MG）作为一种三芳甲烷化合物，对健康构成危害，并引起了相当大的环境关注。在这项工作中，进行了批处理吸附实验，在不同的操作条件下，如 pH 值、接触时间和吸附剂量，以评估酵母生物质从水溶液中去除 MG 染料的最佳参数。然后，采用常规生化分析方法评估酿酒酵母和产朊假丝酵母对 MG 的去除效率（75.18%和 95.85%）。此外，傅里叶变换红外光谱结合拉曼微光谱用于仔细研究两种酵母菌株在去除染料方面的差异。这项研究表明，拉曼微光谱技术可以作为一种有用且强大的工具，用于原位定量测量酵母细胞表面 MG 染料的含量，甚至为寻找从工业废水中去除有毒染料的潜在合适吸附剂提供了一种新的替代技术。
