Liu J H, Zhang L, Zha D C, Chen L Q, Chen X X, Qi Z M
College of Life and Health Science, Anhui Science and Technology University, Fengyang, China.
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, China.
Lett Appl Microbiol. 2018 Oct;67(4):348-353. doi: 10.1111/lam.13043. Epub 2018 Aug 22.
Microalgae have emerged as promising biosorbents for the treatment of malachite green (MG) in wastewater. However, the underlying mechanism for the biosorption of MG onto microalgae is still unclear and needs further intensive study. In this work, synchrotron Fourier-transform infrared (s-FTIR) microspectroscopy in combination with biochemical assay is employed to evaluate MG removal efficiency (95·2%, 75·6% and 66·5%) by three stages of Haematococcus pluvialis. Meanwhile, the various vital changes of algal cells including lipids, proteins, polysaccharides and carotenoids is distinguished and quantified in situ. This study illustrates that s-FTIR microspectroscopy is an effective and powerful tool to scrutinize the mechanism for the interactions between the MG dye and microalgal cells, and it even provides an effective and noninvasive new approach to screen potentially proper biosorbents for the removal of dyes from wastewater.
Microalgae have potential application for their ability to absorb dyes from industrial wastewater. In this study, we initiated the application of synchrotron Fourier-transform infrared (s-FTIR) microspectroscopy to investigate malachite green dye removal efficiency by three stages of Haematococcus pluvialis, demonstrating that s-FTIR is a very powerful tool in exploring the mechanism of the biosorption of dyes onto microalgae.
微藻已成为处理废水中孔雀石绿（MG）的有前景的生物吸附剂。然而，MG在微藻上的生物吸附潜在机制仍不清楚，需要进一步深入研究。在这项工作中，同步辐射傅里叶变换红外（s-FTIR）显微光谱结合生化分析被用于评估雨生红球藻三个阶段对MG的去除效率（分别为95.2%、75.6%和66.5%）。同时，藻细胞中包括脂质、蛋白质、多糖和类胡萝卜素在内的各种重要变化被原位区分和定量。本研究表明，s-FTIR显微光谱是审视MG染料与微藻细胞间相互作用机制的有效且强大的工具，甚至为筛选潜在合适的生物吸附剂以去除废水中的染料提供了一种有效且非侵入性的新方法。
微藻因其从工业废水中吸收染料的能力而具有潜在应用价值。在本研究中，我们首次应用同步辐射傅里叶变换红外（s-FTIR）显微光谱来研究雨生红球藻三个阶段对孔雀石绿染料的去除效率，证明了s-FTIR在探索染料在微藻上生物吸附机制方面是一种非常强大的工具。
