Department of Physiology, Serampore College, Hooghly, India.
Department of Physiology, Raja Peary Mohan College, Hooghly, India.
Int J Radiat Biol. 2019 Nov;95(11):1529-1542. doi: 10.1080/09553002.2019.1642545. Epub 2019 Jul 26.
Evaluation of the modulatory effect of ethanolic extract of tuber (EEAIT) against γ-irradiation induced ovarian and uterine toxicity. Extract preparation was done by 80% hydro-ethanol using Soxhlet apparatus. EEAIT was administered to female Swiss albino mice ( 5) daily (200 and 400 mg/kg body weight/d) for 7 days before γ-irradiation exposure (2.9 Gy). FSH, LH, estrogen, progesterone, cytokine levels, and oxidative stress parameters were measured after 24 hours of γ-irradiation. Histology, folliculogenesis, viability of granulosa cells, ROS measurement by flow cytometry, western blot of P450scc, P45017A1, 3β HSD and SF 1 were also performed. In addition, fertility status was assessed by fecundability and fecundity. The results showed that EEAIT exhibit a strong radioprotective activity by reducing the oxidative stress and thereby restored the ovarian and uterine alterations. EEAIT also improved the abnormality in follicle development, restored altered gonadal hormones and cytokines levels, increase the fertility status, reducing ROS level of granulosa cells with increasing granulosa cells viability and steroidogenic enzyme activity as compared to control. So EEAIT showed a radioprotective effect on γ-irradiation induced ovarian and uterine damage. Our results suggested that tuber can be a potential radioprotector to prevent female infertility.
评价乙醇提取物（EEAIT）对 γ 射线诱导的卵巢和子宫毒性的调节作用。提取物通过索氏提取器用 80%的水-乙醇制备。EEAIT 以 200 和 400mg/kg 体重/天的剂量每天（共 7 天）对雌性瑞士白化小鼠（5 只）进行给药，然后进行 γ 射线照射（2.9Gy）。在 γ 射线照射后 24 小时测量 FSH、LH、雌激素、孕激素、细胞因子水平和氧化应激参数。还进行了组织学、卵泡发生、颗粒细胞活力、通过流式细胞术测量 ROS、P450scc、P45017A1、3β HSD 和 SF1 的 Western blot。此外，通过生育能力和生育力评估生育状况。结果表明，EEAIT 通过降低氧化应激表现出很强的放射防护活性，从而恢复了卵巢和子宫的改变。EEAIT 还改善了卵泡发育异常，恢复了改变的性腺激素和细胞因子水平，提高了生育能力，降低了颗粒细胞 ROS 水平，增加了颗粒细胞活力和类固醇生成酶活性，与对照组相比。因此，EEAIT 对 γ 射线诱导的卵巢和子宫损伤具有放射防护作用。我们的结果表明， tuber 可能是一种潜在的放射保护剂，可预防女性不孕。
