Pal Swagata, Bhattacharjee Ankita, Mukherjee Sandip, Bhattacharya Koushik, Mukherjee Soumya, Khowala Suman
CSIR-Indian Institute of Chemical Biology, Drug Development and Biotechnology, 4 Raja S. C. Mullick Road, Kolkata 700032, India.
Serampore College, Department of Physiology, Hooghly, West Bengal 712201, India.
Biomed Res Int. 2014;2014:349074. doi: 10.1155/2014/349074. Epub 2014 May 29.
The possible protective role of ethanolic extract of A. indica tuber (EEAIT) in hepatotoxicity and apoptosis of liver caused by alcohol in rats was investigated. Treatment of rats with alcohol (3 g ethanol per kg body weight per day for 15 days intraperitoneally) produced marked elevation of liver biomarkers such as serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (γ-GT), and total bilirubin levels which were reduced by EEAIT in a dose-dependent manner. Furthermore, EEAIT improved antioxidant status (MDA, NO, and GSH) and preserved hepatic cell architecture. Simultaneous supplementation with EEAIT significantly restored hepatic catalase (CAT) and superoxide dismutase (SOD) activity levels towards normal. The studies with biochemical markers were strongly supported by the histopathological evaluation of the liver tissue. EEAIT also attenuated apoptosis and necrosis features of liver cell found in immunohistochemical evaluation. HPLC analysis of the extract showed the presence of three major peaks of which peak 2 (RT: 33.33 min) contains the highest area (%) and UV spectrum analysis identified it as flavonoids. It is therefore suggested that EEAIT can provide a definite protective effect against chronic hepatic injury caused by alcohol in rats, which may mainly be associated with its antioxidative effect.
研究了印度天南星块茎乙醇提取物（EEAIT）对大鼠酒精性肝毒性和肝脏细胞凋亡的潜在保护作用。用酒精处理大鼠（每天每千克体重腹腔注射3克乙醇，持续15天）导致肝脏生物标志物显著升高，如血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、γ-谷氨酰转肽酶（γ-GT）和总胆红素水平，而EEAIT以剂量依赖的方式降低了这些指标。此外，EEAIT改善了抗氧化状态（丙二醛、一氧化氮和谷胱甘肽）并保留了肝细胞结构。同时补充EEAIT可使肝脏过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性水平显著恢复正常。肝脏组织的组织病理学评估有力地支持了生化标志物的研究结果。EEAIT还减轻了免疫组织化学评估中发现的肝细胞凋亡和坏死特征。提取物的高效液相色谱分析显示有三个主要峰，其中峰2（保留时间：33.33分钟）的面积百分比最高，紫外光谱分析将其鉴定为黄酮类化合物。因此，提示EEAIT对大鼠酒精性慢性肝损伤具有明确的保护作用，这可能主要与其抗氧化作用有关。
