Haranaka R, Hasegawa R, Nakagawa S, Sakurai A, Satomi N, Haranaka K
Department of Biochemistry, Nihon University School of Medicine, Tokyo, Japan.
J Biol Response Mod. 1988 Feb;7(1):77-90.
To examine the effects of combination therapy with traditional Chinese preparations (Syô-saiko-tô, Zyûzen-taiho-tô or Cinnamomum cortex) and OK432 or mitomycin C on the antitumor activities and TNF producibility, an investigation was carried out using mice transplanted with Ehrlich or Meth A tumor cells. Development of the transplanted tumors was strongly inhibited by the combination therapy, and the tumor necrosis factor (TNF) producibility was increased with the combination of a traditional Chinese preparation and OK432. A significant negative correlation was observed between the TNF activities and tumor weight, and there was a positive correlation between the TNF activities and spleen weight in the Ehrlich-bearing DDY mice receiving traditional Chinese preparations or OK432. Marked lymphocytosis, hyperplasia, and hypertrophy of Kupffer's cells in the liver were noted in the tumor-bearing DDY mice receiving traditional Chinese preparations or OK432. In two-step carcinogenesis experiments involving treatment with DMBA and TPA to induce skin papillomas, Zyûzen-taiho-tô appeared to be effective in inhibiting carcinogenesis. These results suggest that the antitumor activities and capacity for TNF production of the preparations are probably due in part to stimulation of the reticuloendothelial system, including macrophages, and induction of a host-mediated antitumor substance like TNF as one of the immunopotentiators.
为研究中药制剂（小柴胡汤、十全大补汤或肉桂皮）与OK432或丝裂霉素C联合治疗对肿瘤抑制活性及肿瘤坏死因子（TNF）产生能力的影响，利用接种艾氏腹水瘤或Meth A肿瘤细胞的小鼠进行了研究。联合治疗强烈抑制移植瘤的生长，且中药制剂与OK432联合应用可增加肿瘤坏死因子（TNF）的产生能力。在接种艾氏腹水瘤的DDY小鼠中，接受中药制剂或OK432治疗后，TNF活性与肿瘤重量之间存在显著负相关，TNF活性与脾脏重量之间存在正相关。在接受中药制剂或OK432治疗的荷瘤DDY小鼠中，观察到肝脏库普弗细胞明显的淋巴细胞增多、增生和肥大。在涉及用二甲基苯并蒽（DMBA）和12-O-十四酰佛波醇-13-乙酸酯（TPA）诱导皮肤乳头状瘤的两步致癌实验中，十全大补汤似乎对抑制致癌作用有效。这些结果表明，这些制剂的抗肿瘤活性和TNF产生能力可能部分归因于对包括巨噬细胞在内的网状内皮系统的刺激，以及诱导宿主介导的抗肿瘤物质如TNF作为免疫增强剂之一。
