Ohnishi Y, Fujii H, Hayakawa Y, Sakukawa R, Yamaura T, Sakamoto T, Tsukada K, Fujimaki M, Nunome S, Komatsu Y, Saiki I
Department of Pathogenic Biochemistry, Research Institute for Wakan-yaku, Sugitani, Toyama.
Jpn J Cancer Res. 1998 Feb;89(2):206-13. doi: 10.1111/j.1349-7006.1998.tb00550.x.
We have investigated the inhibitory effect of oral administration of Juzen-taiho-to, a Kampo Japanese herbal medicine, on liver metastasis by the inoculation of a liver-metastatic variant (L5) of murine colon 26 carcinoma cells into the portal vein. Oral administration of Juzen-taiho-to for 7 days before tumor inoculation resulted in dose-dependent inhibition of liver tumor colonies and significant enhancement of survival rate as compared with the untreated control, without side effects. We also found that liver metastasis of L5 cells was enhanced in BALB/c mice pretreated with anti-asialo GM1 serum or 2-chloroadenosine, and in BALB/c nu/nu mice, compared to normal mice. This indicates that NK cells, macrophages, and T-cells play important roles in the prevention of metastasis of tumor cells. Juzen-taiho-to significantly inhibited the experimental liver metastasis of colon 26-L5 cells in mice pretreated with anti-asialo GM1 serum and untreated normal mice, whereas it did not inhibit metastasis in 2-chloroadenosine-pretreated mice or T-cell-deficient nude mice. Oral administration of Juzen-taiho-to activated peritoneal exudate macrophages (PEM) to become cytostatic against the tumor cells. These results show that oral administration of Juzen-taiho-to inhibited liver metastasis of colon 26-L5 cells, possibly through a mechanism mediated by the activation of macrophages and/or T-cells in the host immune system. Thus, Juzen-taiho-to may be efficacious for the prevention of cancer metastasis.
我们研究了口服汉方日本草药十全大补汤对通过将小鼠结肠26癌细胞的肝转移变体（L5）接种到门静脉而发生的肝转移的抑制作用。在接种肿瘤前7天口服十全大补汤，与未治疗的对照组相比，导致肝肿瘤集落呈剂量依赖性抑制且存活率显著提高，且无副作用。我们还发现，与正常小鼠相比，用抗去唾液酸GM1血清或2-氯腺苷预处理的BALB/c小鼠以及BALB/c裸鼠中L5细胞的肝转移增强。这表明自然杀伤细胞、巨噬细胞和T细胞在预防肿瘤细胞转移中起重要作用。十全大补汤显著抑制了用抗去唾液酸GM1血清预处理的小鼠和未治疗的正常小鼠中结肠26-L5细胞的实验性肝转移，而在2-氯腺苷预处理的小鼠或T细胞缺陷的裸鼠中它没有抑制转移。口服十全大补汤可激活腹腔渗出巨噬细胞（PEM），使其对肿瘤细胞具有细胞生长抑制作用。这些结果表明，口服十全大补汤抑制结肠26-L5细胞的肝转移，可能是通过宿主免疫系统中巨噬细胞和/或T细胞的激活介导的机制。因此，十全大补汤可能对预防癌症转移有效。
