转译烟草植物中内几丁质酶基因提高对真菌病原体的耐受性和抗虫性。
Enhanced Tolerance against a Fungal Pathogen and Insect Resistance in Transgenic Tobacco Plants Overexpressing an Endochitinase Gene from .
机构信息
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos 62209, Mexico.
Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos 62209, Mexico.
出版信息
Int J Mol Sci. 2019 Jul 16;20(14):3482. doi: 10.3390/ijms20143482.
In this study we cloned a chitinase gene (), from isolated from the corpse of a lepidopteran, which is an important sugarcane pest. The chitinase gene amplified from the genome was cloned into the transformation vector p2X35SChiC and used to transform tobacco ( L. cv Petit Havana SR1). The resistance of these transgenic plants to the necrotrophic fungus and to the pest was evaluated: both the activity of chitinase as well as the resistance against and was significantly higher in transgenic plants compared to the wild-type.
在这项研究中，我们从鳞翅目昆虫尸体中克隆了一个几丁质酶基因（），该昆虫是甘蔗的重要害虫。从基因组中扩增的几丁质酶基因被克隆到转化载体 p2X35SChiC 中，并用于转化烟草（L. cv Petit Havana SR1）。评估了这些转基因植物对坏死真菌和鳞翅目害虫的抗性：与野生型相比，转基因植物的几丁质酶活性以及对和的抗性均显著提高。
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