Laboratorio de Química y Biotecnología de Productos Lácteos, Centro de Investigación en Alimentación y Desarrollo A. C. (CIAD), Carretera Gustavo Enrique Astiazarán Rosas, No. 46 Col. La Victoria, CP, Hermosillo 83304, Sonora, Mexico.
Laboratorio de Microbiología e Inmunología, Centro de Investigación en Alimentación y Desarrollo A. C. (CIAD), Carretera Gustavo Enrique Astiazarán Rosas, No. 46 Col. La Victoria, CP, Hermosillo 83304, Sonora, Mexico.
Nutrients. 2019 Jul 16;11(7):1610. doi: 10.3390/nu11071610.
The aim of this study was to evaluate the effect of milk fermented with J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin + FMJ28, and untreated (control). Groups were fed fermented milk for 15 days, followed by administration of indomethacin supplied in three sub-doses over experimental period. Body weight, and food consumption were recorded. Additionally, spleen, kidney, and liver were weighed, and the small intestine length was measured. The cytokines in serum (IL-2, IL-4, IL-6, IL-10, IL-17, IL-23 and TNFα) and in intestinal mucosa (IL-17 and IFNγ) were also determined. Compared to the control, all indomethacin-supplemented groups lost weight (~2.7 g; < 0.05), but no changes were found in the organ-specific morphometry analysis. FMJ28 showed better results in attenuating serum and intestinal IL-17 levels. Furthermore, showed less epithelial cell loss and inflammatory infiltrates than the other indomethacin-treated groups. These results suggest that FMJ28 may be effective in reducing intestinal and systemic acute inflammation, specifically in mice.
本研究旨在评估 J20（FMJ20）或 J28（FMJ28）发酵奶对改善消炎痛诱导的炎症的作用。将 28 只雄性 C57Bl/6 小鼠分为四组：消炎痛组、消炎痛+FMJ20 组、消炎痛+FMJ28 组和未处理（对照）组。各组均喂食发酵奶 15 天，然后在实验期间给予三剂消炎痛。记录体重和食物摄入量。此外，还测量了脾脏、肾脏和肝脏的重量以及小肠的长度。还测定了血清（IL-2、IL-4、IL-6、IL-10、IL-17、IL-23 和 TNFα）和肠黏膜（IL-17 和 IFNγ）中的细胞因子。与对照组相比，所有给予消炎痛的组体重均减轻（~2.7 g；<0.05），但器官特异性形态计量分析无变化。FMJ28 对减轻血清和肠道 IL-17 水平的效果更好。此外，与其他消炎痛治疗组相比，FMJ28 组上皮细胞丢失和炎症浸润较少。这些结果表明，FMJ28 可能对减轻肠道和全身急性炎症有效，尤其是在小鼠中。
