School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan.
Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan.
Nutrients. 2019 Apr 27;11(5):969. doi: 10.3390/nu11050969.
Inflammatory bowel disease (IBD) is an emerging health problem associated with the dysregulation of the intestinal immune system and microbiome. Probiotics are able to reduce inflammatory responses in intestinal epithelial cells (IECs). However, entire signaling pathways and the interaction between different probiotics have not been well-documented. This study was designed to investigate the anti-inflammatory effects and mechanisms of single and combined probiotics. HT-29 cells were induced by lipopolysaccharide (LPS) and tumor necrosis factor (TNF)-α, treated with , subsp. or their combination and analyzed for inflammation-related molecules. Both and subsp. reduced interleukin (IL)-8 secretion and the expressions of phosphorylated p65 nuclear factor-kappa B (-p65 NF-κB), phosphorylated p38 mitogen-activated protein kinase (-p38 MAPK), vascular cell adhesion molecule-1 (VCAM-1) and cyclooxygenase-2 (COX-2), while they increased toll-like receptor 2 (TLR2) expression. did not decrease intercellular adhesion molecule-1 (ICAM-1) but enhanced the inhibitory efficacy of subsp. . Combined probiotics showed the best anti-inflammatory activity. These results suggest that and subsp. may exert a potent anti-inflammatory effect through modulating TLR2-mediated NF-κB and MAPK signaling pathways in inflammatory IECs. Both strains, especially their combination, may be novel adjuvants for IBD therapy.
炎症性肠病（IBD）是一种与肠道免疫系统和微生物群失调相关的新兴健康问题。益生菌能够减少肠道上皮细胞（IECs）的炎症反应。然而，整个信号通路和不同益生菌之间的相互作用尚未得到很好的记录。本研究旨在研究单一和组合益生菌的抗炎作用和机制。用脂多糖（LPS）和肿瘤坏死因子（TNF）-α诱导 HT-29 细胞，用 、 subsp. 或其组合处理，并分析与炎症相关的分子。 和 subsp. 均降低白细胞介素（IL）-8 的分泌和磷酸化 p65 核因子-κB（-p65 NF-κB）、磷酸化 p38 丝裂原活化蛋白激酶（-p38 MAPK）、血管细胞黏附分子-1（VCAM-1）和环氧化酶-2（COX-2）的表达，同时增加 Toll 样受体 2（TLR2）的表达。 不会降低细胞间黏附分子-1（ICAM-1），但增强了 subsp. 的抑制作用。组合益生菌表现出最佳的抗炎活性。这些结果表明， 和 subsp. 可能通过调节 TLR2 介导的 NF-κB 和 MAPK 信号通路在炎症性 IECs 中发挥强大的抗炎作用。两种菌株，尤其是它们的组合，可能是治疗 IBD 的新型佐剂。
